Evidence map›Paper›PMID 34189615›Full record

ArticleApplied microbiology and biotechnology2021

Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase.

Andreas Kruschitz, Linda Peinsipp, Martin Pfeiffer, Bernd Nidetzky

Open access · hybridAbstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Andreas KruschitzAustrian Centre of Industrial Biotechnology (acib), Krenngasse 37, 8010, Graz, Austria.
Linda PeinsippAustrian Centre of Industrial Biotechnology (acib), Krenngasse 37, 8010, Graz, Austria.
Martin PfeifferAustrian Centre of Industrial Biotechnology (acib), Krenngasse 37, 8010, Graz, Austria.
Bernd NidetzkyAustrian Centre of Industrial Biotechnology (acib), Krenngasse 37, 8010, Graz, Austria. bernd.nidetzky@tugraz.at.ORCID http://orcid.org/0000-0002-5030-2643
Graz University of Technology · AT

Funding

European Commission No 761030 (CARBAFIN)
6 · The paper itself

Abstract

Advanced biotransformation processes typically involve the upstream processing part performed continuously and interlinked tightly with the product isolation. Key in their development is a catalyst that is highly active, operationally robust, conveniently produced, and recyclable. A promising strategy to obtain such catalyst is to encapsulate enzymes as permeabilized whole cells in porous polymer materials. Here, we show immobilization of the sucrose phosphorylase from Bifidobacterium adolescentis (P134Q-variant) by encapsulating the corresponding E. coli cells into polyacrylamide. Applying the solid catalyst, we demonstrate continuous production of the commercial extremolyte 2-α-D-glucosyl-glycerol (2-GG) from sucrose and glycerol. The solid catalyst exhibited similar activity (≥70%) as the cell-free extract (~800 U g

Indexed as

GlucosidesSucroseEscherichia coliGlucosyltransferasesTechnologyGlucosidesGlucosyltransferasesSucrosesucrose phosphorylase2-α-D-Glucosyl-glycerolContinuous biomanufacturingFlow bio-catalysisPacked-bed reactorSucrose phosphorylaseWhole cell-based enzyme immobilization

Identifiers

PMID34189615
PMCPMC8285329
OpenAlexW3175076014

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.