Evidence map›Paper›PMID 34189474›Full record

ArticleSTAR protocols2021

Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation.

Franziska Greulich, Aikaterini Mechtidou, Teresa Horn, Nina Henriette Uhlenhaut

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Franziska GreulichMetabolic Programming, School of Life Sciences Weihenstephan, ZIEL-Institute for Food & Health, Technische Universitaet Muenchen (TUM), 85354 Freising, Germany.
Aikaterini MechtidouInstitute for Diabetes and Obesity (IDO) & Institute for Diabetes and Cancer (IDC), Helmholtz Center Munich (HMGU) and German Center for Diabetes Research (DZD), 85764 Neuherberg (Munich), Germany.
Teresa HornInstitute for Diabetes and Obesity (IDO) & Institute for Diabetes and Cancer (IDC), Helmholtz Center Munich (HMGU) and German Center for Diabetes Research (DZD), 85764 Neuherberg (Munich), Germany.
Nina Henriette UhlenhautMetabolic Programming, School of Life Sciences Weihenstephan, ZIEL-Institute for Food & Health, Technische Universitaet Muenchen (TUM), 85354 Freising, Germany.
Deutsches Diabetes-Zentrum e.V. · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from

Indexed as

AnimalsBinding SitesChromatin ImmunoprecipitationDrosophila melanogasterHigh-Throughput Nucleotide SequencingMiceProteinsReal-Time Polymerase Chain ReactionProteinsCell BiologyChIPseqChromatin immunoprecipitation (ChIP)Molecular BiologySequence analysis

Identifiers

PMID34189474
PMCPMC8220248
OpenAlexW3167739889

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.