ArticleSTAR protocols2021
Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Labeling Postsynaptic Densities for Super-Resolution Microscopy With Minimal Signal-Loss and Offset.Bio-protocol · 2025Article
- Aggregation-Dependent Epitope Sequence and Modification Fingerprints of Anti-Aβ Antibodies.bioRxiv : the preprint server for biology · 2025Article
- High-Throughput Tiling of Essential mRNAs Increases Potency of Antisense Antibiotics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Novel High-Throughput Electrochemical Detection of Staphylococcus Aureus, Bacillus Cereus, or Micrococcus Luteus Using AuNPs@TiSmall (Weinheim an der Bergstrasse, Germany) · 2025Article
- Facile High-Throughput Pre-purification of Biological Samples for Library-Scale Peptide Interaction Studies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Molecular dissection of an immunodominant epitope in KFrontiers in immunology · 2024Article
- Human antibody profiling technologies for autoimmune disease.Immunologic research · 2023Review
- Molecular characterization of AIFM2/FSP1 inhibition by iFSP1-like molecules.Cell death & disease · 2023Article
- Multivalent binding kinetics resolved by fluorescence proximity sensing.Communications biology · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-peptide interactions are involved in many fundamental cellular functions and constitute promising drug targets. Here, we provide a detailed protocol for the cost-effective preparation of a cellulose-based solid support for synthesis of nanoscale to micromolar-scale peptide libraries. Their subsequent use for high-throughput protein interaction screening as well as affinity determination in solution provides binding data for thousands of unique peptides with a turnover of 1 to 2 weeks, thereby facilitating
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Registered trials
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