Evidence map›Paper›PMID 34189467›Full record

ArticleSTAR protocols2021

Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.

Barbara K Stepien, Samir Vaid, Ronald Naumann, Anja Holtz, Wieland B Huttner

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Barbara K StepienMax Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Samir VaidMax Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Ronald NaumannMax Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Anja HoltzMax Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Wieland B HuttnerMax Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Max Planck Institute of Molecular Cell Biology and Genetics · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Donor embryonic stem cells of one species are microinjected into early embryos of the other species (recipient), which are implanted into host foster mothers of the recipient species. For complete details on the use and execution of this protocol, please refer to Stepien et al. (2020).

Indexed as

ChimeraEmbryo, MammalianAnimalsBlastocystEmbryonic Stem CellsFemaleMaleMiceMicroinjectionsRatsCell BiologyCell cultureCell isolationModel OrganismsNeuroscienceStem Cells

Identifiers

PMID34189467
PMCPMC8219896
OpenAlexW3169644047

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.