Evidence map›Paper›PMID 34189438›Full record

ArticleiScience2021

Modelling TGFβR and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer.

Robin M Delaine-Smith, Eleni Maniati, Beatrice Malacrida, Sam Nichols, Reza Roozitalab, Roanne R Jones, Laura S M Lecker, Oliver M T Pearce, Martin M Knight, Frances R Balkwill

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 24 citations in OpenAlex.

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  17. BuildingSTAR protocols · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Robin M Delaine-SmithBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Eleni ManiatiBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Beatrice MalacridaBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Sam NicholsBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Reza RoozitalabBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Roanne R JonesBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Laura S M LeckerBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Oliver M T PearceBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Martin M KnightInstitute of Bioengineering and School of Engineering and Materials Science, Queen Mary University of London, Mile End Road E1, London, UK.
Frances R BalkwillBarts Cancer Institute, Queen Mary University of London, Charterhouse Square EC1M 6BQ, London, UK.
Queen Mary University of London · GB

Funding

Cancer Research UK 16354Cancer Research UK 25714Cancer Research UK 27947
6 · The paper itself

Abstract

In a multi-level "deconstruction" of omental metastases, we previously identified a prognostic matrisome gene expression signature in high-grade serous ovarian cancer (HGSOC) and twelve other malignancies. Here, our aim was to understand how six of these extracellular matrix (ECM) molecules, COL11A1, cartilage oligomeric matrix protein, FN1, versican, cathepsin B, and COL1A1, are upregulated in cancer. Using biopsies, we identified significant associations between TGFβR activity, Hedgehog (Hh) signaling, and these ECM molecules and studied the associations in mono-, co-, and tri-culture. Activated omental fibroblasts (OFs) produced more matrix than malignant cells, directed by TGFβR and Hh signaling cross talk. We "reconstructed" omental metastases in tri-cultures of HGSOC cells, OFs, and adipocytes. This combination was sufficient to generate all six ECM proteins and the matrisome expression signature. TGFβR and Hh inhibitor combinations attenuated fibroblast activation and gel and ECM remodeling in these models. The tri-culture model reproduces key features of omental metastases and allows study of diseased-associated ECM.

Indexed as

CancerMolecular biologyTissue engineering

Identifiers

PMID34189438
PMCPMC8215304
OpenAlexW3172175489

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.