ArticleEcology and evolution2021
Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks.
Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Fish Diversity and Functional Traits in the Seagrass Based on the Environmental DNA Metabarcoding in the Li'an Bay, China.Animals : an open access journal from MDPI · 2026Article
- Refinement and application of 12SCurrent research in food science · 2026Article
- A simple and cost-effective extraction for piscine environmental DNA metabarcoding using guanidine hydrochloride method.MethodsX · 2024Article
- Experimental evaluation of genetic variability based on DNA metabarcoding from the aquatic environment: Insights from the LerayEcology and evolution · 2024Article
- Application of environmental DNA metabarcoding to identify fish community characteristics in subtropical river systems.Ecology and evolution · 2024Article
- Diversity and spatiotemporal dynamics of fish communities in the Chongqing section of the upper Yangtze River based on eDNA metabarcoding.Ecology and evolution · 2023Article
- Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary.PloS one · 2022Article
- Article
- Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks.Ecology and evolution · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA (eDNA) techniques refer to utilizing the organisms' DNA extracted from environment samples to genetically identify target species without capturing actual organisms. eDNA metabarcoding via high-throughput sequencing can simultaneously detect multiple fish species from a single water sample, which is a powerful tool for the qualitative detection and quantitative estimates of multiple fish species. However, sequence counts obtained from eDNA metabarcoding may be influenced by many factors, of which primer bias is one of the foremost causes of methodological error. The performance of 18 primer pairs for
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