ArticleEcology and evolution2021
Relieving efforts in palm-tree tissue sampling for population genetics analyses.
Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Relieving efforts in palm-tree tissue sampling for population genetics analyses.Ecology and evolution · 2021Article
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1 author.
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Abstract
The young leaves are the main source of nucleic acids for population genetic studies in palm-trees; however, the access to this tissue may be limited by specific features of each species. Using root tissues as an alternative source of nucleic acids could facilitate the sampling in large populations.This study tests root tissue viability as an alternative nucleic acid source (root versus. leaf) and explores different protocols (tissue storage and DNA extraction methods) to obtain high-quality DNA samples.The results showed no significant differences in DNA concentration (603.7 vs. 599.1 ng/μl) and quality ratios (A260/280:2.1 vs. 1.9, and A260/230:2.1 vs. 2.0) for the comparisons of tissue source (leaf vs. root) and DNA extraction method (manual vs. kit). For tissue storage method, DNA concentration was significantly higher for root tissues stored in 70% and 90% alcohol solutions (692.8 and 822.6 ng/μl, respectively) versus those obtained from leaf tissue (603.7 ng/μl); however, for the quality parameters, no differences were found.Results showed the effective potential of using root tissue as an alternative source for nucleic acids, which could facilitate population sampling of palm-tree species for future studies, and this methodological alternative could be applied to other plant systems with similar sampling challenges. .
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