ArticleScientific reports2021
Development of a bioavailable Hg(II) sensing system based on MerR-regulated visual pigment biosynthesis.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Replacing Manual Operation with Bio-Automation II: Construction of a Biological Digestion Gene Circuit to Eliminate the Interference of Food Matrices in the Rapid Detection of Heavy Metals.Foods (Basel, Switzerland) · 2025Article
- Pyomelanin-powered whole-cell biosensor for ultrasensitive and selective detection of bioavailable Hg(ii).RSC advances · 2025Article
- Whole Cells of Microorganisms-A Powerful Bioanalytical Tool for Measuring Integral Parameters of Pollution: A Review.Biosensors · 2025Review
- Microbial Transcription Factor-Based Biosensors: Innovations from Design to Applications in Synthetic Biology.Biosensors · 2025Review
- Systematic evaluation of pigment-based whole-cell lead biosensors: challenges in genetic circuit engineering and critical considerations for background noise control.Frontiers in bioengineering and biotechnology · 2025Article
- Biosynthesis of Indigo Dyes and Their Application in Green Chemical and Visual Biosensing for Heavy Metals.ACS omega · 2024Article
- Pigments from pathogenic bacteria: a comprehensive update on recent advances.World journal of microbiology & biotechnology · 2024Review
- Whole-Cell Biosensor for Iron Monitoring as a Potential Tool for Safeguarding Biodiversity in Polar Marine Environments.Marine drugs · 2024Review
- Article
- Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains.Bioprocess and biosystems engineering · 2024Article
- NeMeHg, genetically encoded indicator for mercury ions based on mNeonGreen green fluorescent protein and merP protein fromFrontiers in bioengineering and biotechnology · 2024Article
- Synthetic bacteria for the detection and bioremediation of heavy metals.Frontiers in bioengineering and biotechnology · 2023Review
- Transcription factor-based biosensors for screening and dynamic regulation.Frontiers in bioengineering and biotechnology · 2023Review
- Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead.Frontiers in microbiology · 2023Article
- Biological and technical challenges for implementation of yeast-based biosensors.Microbial biotechnology · 2023Review
- Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor.RSC advances · 2022Article
- Detection of environmental pollutant cadmium in water using a visual bacterial biosensor.Scientific reports · 2022Article
- Anthocyanin biosynthetic pathway switched by metalloregulator PbrR to enable a biosensor for the detection of lead toxicity.Frontiers in microbiology · 2022Article
- Differential Detection of Bioavailable Mercury and Cadmium Based on a Robust Dual-Sensing Bacterial Biosensor.Frontiers in microbiology · 2022Article
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.World journal of microbiology & biotechnology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered microorganisms have proven to be a highly effective and robust tool to specifically detect heavy metals in the environment. In this study, a highly specific pigment-based whole-cell biosensor has been investigated for the detection of bioavailable Hg(II) based on an artificial heavy metal resistance operon. The basic working principle of biosensors is based on the violacein biosynthesis under the control of mercury resistance (mer) promoter and mercury resistance regulator (MerR). Engineered biosensor cells have been demonstrated to selectively respond to Hg(II), and the specific response was not influenced by interfering metal ions. The response of violacein could be recognized by the naked eye, and the time required for the maximum response of violacein (5 h) was less than that of enhanced green fluorescence protein (eGFP) (8 h) in the single-signal output constructs. The response of violacein was almost unaffected by the eGFP in a double-promoter controlled dual-signals output construct. However, the response strength of eGFP was significantly decreased in this genetic construct. Exponentially growing violacein-based biosensor detected concentrations as low as 0.39 μM Hg(II) in a colorimetric method, and the linear relationship was observed in the concentration range of 0.78-12.5 μM. Non-growing biosensor cells responded to concentrations as low as 0.006 μM Hg(II) in a colorimetric method and in a Hg(II) containing plate sensitive assay, and the linear relationship was demonstrated in a very narrow concentration range. The developed biosensor was finally validated for the detection of spiked bioavailable Hg(II) in environmental water samples.
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