ArticleBreast cancer research : BCR2021
Single cell transcriptome atlas of mouse mammary epithelial cells across development.
Article in Breast cancer research : BCR, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 70 citations in OpenAlex.
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- Pregnancy-induced tissue-resident memory-like T cells contribute to tumor control in breast cancer.Nature immunology · 2026Article
- Integrated Single-Nucleus Multi-Omics Atlases Reveal Lineage Plasticity and Regulatory Networks of Luminal Epithelial Cells During Mammary Gland Lactation and Involution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Discovery and Evaluation of Biomarkers for Triple-Negative Breast Cancer Subtypes Uncovers Patient Stratification and Targeted Therapeutic Strategies.Cancer research · 2026Article
- Branching out: the diverse roles of stem and progenitor cells in mammary gland development.Journal of mammary gland biology and neoplasia · 2026Review
- Single-cell transcriptome sequencing revealing the microenvironment of breast fibroepithelial tumor.Biochemistry and biophysics reports · 2026Article
- The mTOR signaling pathway regulates key steps of mammary gland organoid genesis in a temporal manner.Scientific reports · 2026Article
- Divergent aging of nulliparous and parous mammary glands reveals IL33+ hybrid epithelial cells.Nature communications · 2026Article
- RNA-seq dataset of the estrogen-dependent regulation of the transcriptome in mouse mammary gland organoids.Data in brief · 2025Article
- Contractile fibroblasts form a transient niche for the branching mammary epithelium.Nature communications · 2025Article
- Defining breast epithelial cell types in the single-cell era.Developmental cell · 2025Review
- Single-cell analysis of gene regulatory networks in the mammary glands of P4HA1-knockout mice.PLoS genetics · 2025Article
- Transcriptional landscapes underlying Notch-induced lineage conversion and plasticity of mammary basal cells.The EMBO journal · 2025Article
- Distinct mammary stem cells orchestrate long-term homeostasis of adult mammary gland.Cell discovery · 2025Article
- CytoSimplex: visualizing single-cell fates and transitions on a simplex.Bioinformatics (Oxford, England) · 2025Article
- Neutrophils physically interact with tumor cells to form a signaling niche promoting breast cancer aggressiveness.Nature cancer · 2025Article
- Transgenic overexpression of the miR-200b/200a/429 cluster prevents mammary tumor initiation in Neu/Erbb2 transgenic mice.International journal of cancer · 2025Article
- Benchmarking cell type annotation methods for 10x Xenium spatial transcriptomics data.BMC bioinformatics · 2025Article
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundHeterogeneity within the mouse mammary epithelium and potential lineage relationships have been recently explored by single-cell RNA profiling. To further understand how cellular diversity changes during mammary ontogeny, we profiled single cells from nine different developmental stages spanning late embryogenesis, early postnatal, prepuberty, adult, mid-pregnancy, late-pregnancy, and post-involution, as well as the transcriptomes of micro-dissected terminal end buds (TEBs) and subtending ducts during puberty.
methodsThe single cell transcriptomes of 132,599 mammary epithelial cells from 9 different developmental stages were determined on the 10x Genomics Chromium platform, and integrative analyses were performed to compare specific time points.
resultsThe mammary rudiment at E18.5 closely aligned with the basal lineage, while prepubertal epithelial cells exhibited lineage segregation but to a less differentiated state than their adult counterparts. Comparison of micro-dissected TEBs versus ducts showed that luminal cells within TEBs harbored intermediate expression profiles. Ductal basal cells exhibited increased chromatin accessibility of luminal genes compared to their TEB counterparts suggesting that lineage-specific chromatin is established within the subtending ducts during puberty. An integrative analysis of five stages spanning the pregnancy cycle revealed distinct stage-specific profiles and the presence of cycling basal, mixed-lineage, and 'late' alveolar intermediates in pregnancy. Moreover, a number of intermediates were uncovered along the basal-luminal progenitor cell axis, suggesting a continuum of alveolar-restricted progenitor states.
conclusionsThis extended single cell transcriptome atlas of mouse mammary epithelial cells provides the most complete coverage for mammary epithelial cells during morphogenesis to date. Together with chromatin accessibility analysis of TEB structures, it represents a valuable framework for understanding developmental decisions within the mouse mammary gland.
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