ArticleMolecular brain2021
In vivo imaging of immediate early gene expression dynamics segregates neuronal ensemble of memories of dual events.
Article in Molecular brain, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Let's talk about sex: Male and female mice show similar fear memory retention despite hippocampal activity differences during encoding and consolidation.bioRxiv : the preprint server for biology · 2026Article
- Epigenome regulators imbue a single eukaryotic promoter with diverse gene expression dynamics.iScience · 2026Article
- Place field dynamics in retrosplenial cortex compared to hippocampus.Progress in neurobiology · 2026Article
- Melt electrowritten medium chain length polyhydroxyalkanoate cardiac patches for Post-MI cardiac regeneration.Materials today. Bio · 2025Article
- Regulation ofInternational journal of molecular sciences · 2025Review
- Combinative Protein Expression of Immediate Early Genes c-Fos, Arc, and Npas4 Along Aversive and Appetitive Experience-Related Neural Networks.Hippocampus · 2025Article
- Simultaneous RNA Sequencing and DNA Methylation Profiling Reveals Neural Mechanisms That Regulate Sensitive Period Behavioral Learning.Genes, brain, and behavior · 2025Article
- Hyperactive delta isoform of PI3 kinase enables long-distance regeneration of adult rat corticospinal tract.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Event boundaries drive norepinephrine release and distinctive neural representations of space in the rodent hippocampus.bioRxiv : the preprint server for biology · 2024Article
- Spatially Resolved Transcriptomic Signatures of Hippocampal Subregions andbioRxiv : the preprint server for biology · 2024Article
- Spatially resolved transcriptomic signatures of hippocampal subregions andFrontiers in molecular neuroscience · 2024Article
- Neuronal membrane proteasome-derived peptides modulate NMDAR-dependent neuronal signaling to promote changes in gene expression.Molecular biology of the cell · 2024Article
- Early life adversity shapes social subordination and cell type-specific transcriptomic patterning in the ventral hippocampus.Science advances · 2023Article
- M3 Receptor Pathway Stimulates Rapid Transcription of the CB1 Receptor Activation through Calcium Signalling and the CNR1 Gene Promoter.International journal of molecular sciences · 2023Article
- Real-time visualization of mRNA synthesis during memory formation in live mice.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A new paradigm for investigating temporal order memory shows higher order associations are present in recent but not in remote retrieval.Experimental brain research · 2022Article
- Fluorescent transgenic mouse models for whole-brain imaging in health and disease.Frontiers in molecular neuroscience · 2022Review
- Retrosplenial cortex in spatial memory: focus on immediate early genes mapping.Molecular brain · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of neurons undergoing plasticity in response to external stimuli is one of the pertinent problems in neuroscience. Immediate early genes (IEGs) are widely used as a marker for neuronal plasticity. Here, we model the dynamics of IEG expression as a consecutive, irreversible first-order reaction with a limiting substrate. First, we develop an analytical framework to show that such a model, together with two-photon in vivo imaging of IEG expression, can be used to identify distinct neuronal subsets representing multiple memories. Using the above combination, we show that the expression kinetics, rather than intensity threshold, can be used to identify neuronal ensembles responding to the presentation of two events in vivo. The analytical expression allowed us to segregate the neurons based on their temporal response to one specific behavioural event, thereby improving the ability to detect plasticity related neurons. We image the retrosplenial cortex (RSc) of cfos-GFP transgenic mice to follow the dynamics of cellular changes resulting from contextual fear conditioning behaviour, enabling us to establish a representation of context in RSc at the cellular scale following memory acquisition. Thus, we obtain a general method that distinguishes neurons that took part in multiple temporally separated events by measuring fluorescence of individual neurons in live mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.