ArticleMicrobial cell factories2021
Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Advances in glycerol metabolism comprehension in yeasts: from regulation to metabolic engineering strategies.World journal of microbiology & biotechnology · 2026Review
- 1,3-propanediol production by Klebsiella pneumoniae ∆dhaM∆ptsG∆glpK using glucose and glycerol as co-substrates.Microbial cell factories · 2025Article
- Biotechnological production of 2-keto-D-gluconic acid (2-KGA): from strain engineering to process development.Biotechnology letters · 2025Review
- Impact of nitric oxide donors on capsule, biofilm and resistance profiles of Klebsiella pneumoniae.International journal of antimicrobial agents · 2024Article
- Optimizing enzyme properties to enhance dihydroxyacetone production via methylglyoxal biosensor development.Microbial cell factories · 2024Article
- Enhancing the capability of Klebsiella pneumoniae to produce 1, 3-propanediol by overexpression and regulation through CRISPR-dCas9.Microbial biotechnology · 2022Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundKlebsiella pneumoniae is a bacterium that can be used as producer for numerous chemicals. Glycerol can be catabolised by K. pneumoniae and dihydroxyacetone is an intermediate of this catabolism pathway. Here dihydroxyacetone and glycerol were produced from glucose by this bacterium based a redirected glycerol catabolism pathway.
resultstpiA, encoding triosephosphate isomerase, was knocked out to block the further catabolism of dihydroxyacetone phosphate in the glycolysis. After overexpression of a Corynebacterium glutamicum dihydroxyacetone phosphate dephosphorylase (hdpA), the engineered strain produced remarkable levels of dihydroxyacetone (7.0 g/L) and glycerol (2.5 g/L) from glucose. Further increase in product formation were obtained by knocking out gapA encoding an iosenzyme of glyceraldehyde 3-phosphate dehydrogenase. There are two dihydroxyacetone kinases in K. pneumoniae. They were both disrupted to prevent an inefficient reaction cycle between dihydroxyacetone phosphate and dihydroxyacetone, and the resulting strains had a distinct improvement in dihydroxyacetone and glycerol production. pH 6.0 and low air supplement were identified as the optimal conditions for dihydroxyacetone and glycerol production by K, pneumoniae ΔtpiA-ΔDHAK-hdpA. In fed batch fermentation 23.9 g/L of dihydroxyacetone and 10.8 g/L of glycerol were produced after 91 h of cultivation, with the total conversion ratio of 0.97 mol/mol glucose.
conclusionsThis study provides a novel and highly efficient way of dihydroxyacetone and glycerol production from glucose.
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