Evidence map›Paper›PMID 34187456›Full record

ArticleBMC complementary medicine and therapies2021

Identification of 3,3'-O-dimethylellagic acid and apigenin as the main antiplasmodial constituents of Endodesmia calophylloides Benth and Hymenostegia afzelii (Oliver.) Harms.

Rodrigue Keumoe, Jean Garba Koffi, Darline Dize, Patrick Valère Tsouh Fokou, Joseph Tchamgoue, Lawrence Ayong, Bruno Lenta Ndjakou, Norbert Sewald, Bathelemy Ngameni, Fabrice Fekam Boyom

Abstract read
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In one paragraph

Article in BMC complementary medicine and therapies, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rodrigue KeumoeAntimicrobial and Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Jean Garba KoffiHigher Teachers Training College, University of Yaoundé I, P.O Box 47, Yaounde, Cameroon.
Darline DizeAntimicrobial and Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Patrick Valère Tsouh FokouAntimicrobial and Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Joseph TchamgoueHigher Teachers Training College, University of Yaoundé I, P.O Box 47, Yaounde, Cameroon.
Lawrence AyongMalaria Research Unit, Centre Pasteur du Cameroun, P.O. Box 1274, Yaoundé, Cameroon.
Bruno Lenta NdjakouHigher Teachers Training College, University of Yaoundé I, P.O Box 47, Yaounde, Cameroon.
Norbert SewaldOrganic and Bioorganic Chemistry, Faculty of Chemistry, Bielefeld University, D-33501, Bielefeld, Germany.
Bathelemy NgameniLaboratory of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O Box 1364, Yaounde, Cameroon.
Fabrice Fekam BoyomAntimicrobial and Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. fabrice.boyom@fulbrightmail.org.

Funding

YaBiNaPA N°57316173
6 · The paper itself

Abstract

backgroundEndodesmia calophylloides and Hymenostegia afzelii belong to the Guttiferae and Caesalpiniaceae plant families with known uses in African ethno-medicine to treat malaria and several other diseases. This study aimed at identifying antiplasmodial natural products from selected crude extracts from H. afzelii and E. calophylloides and to assess their cytotoxicity.

methodsThe extracts from H. afzelii and E. calophylloides were subjected to bioassay-guided fractionation to identify antiplasmodial compounds. The hydroethanol and methanol stem bark crude extracts, fractions and isolated compounds were assessed for antiplasmodial activity against the chloroquine-sensitive 3D7 and multi-drug resistant Dd2 strains of Plasmodium falciparum using the SYBR green I fluorescence-based microdilution assay. Cytotoxicity of active extracts, fractions and compounds was determined on African green monkey normal kidney Vero and murine macrophage Raw 264.7 cell lines using the Resazurin-based viability assay.

resultsThe hydroethanolic extract of H. afzelii stem bark (Hasb

conclusionsThis study led to the identification of two potent antiplasmodial natural compounds, 3,3'-O-dimethylellagic acid and apigenin that could serve as starting points for further antimalarial drug discovery.

Indexed as

AnimalsAntimalarialsApigeninCell LineChlorocebus aethiopsEllagic AcidMacrophagesMicePlant BarkPlant ExtractsPlasmodium falciparumVero CellsAntimalarialsApigeninEllagic AcidPlant Extracts3,3′-O-dimethylellagic acidAntiplasmodial activityApigeninBioguided fractionation

Identifiers

PMID34187456
PMCPMC8243547

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.