ArticlePLoS pathogens2021
Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection.
Article in PLoS pathogens, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Single-cell RNA sequencing offers novel perspectives in viral infection research.Frontiers in cellular and infection microbiology · 2026Pooled it
- Zebrafish larvae reveal early neuropathology and host responses to nervous necrosis virus.iScience · 2026Article
- LncRNA-miRNA-mRNA Regulatory Network Reveals Potential Immune Responses in Larval Tomato Hind (Biology · 2026Article
- Single-cell transcriptome profiling reveals herpesviral manipulation of host processes in spleen of gibel carp infected with a Cyvirus cyprinidallo2.PLoS pathogens · 2026Article
- Lipidomics reveals the pro-viral roles of ceramides during fish nodavirus infection.Journal of virology · 2026Article
- Single-nucleus RNA sequencing reveals the underlying roadmap of early gonadal differentiation in teleost.Science China. Life sciences · 2026Article
- Single-Nucleus Transcriptional Profiling Revealed Cell Diversity and Albino Mutation Mechanism in the Skin ofInternational journal of molecular sciences · 2026Article
- Chemosensory protein 3 is a brain host factor for the induction of enhanced-locomotory activity in the BmNPV-silkworm infection model.PLoS pathogens · 2025Article
- Single-Cell Transcriptome Profiling Reveals Conserved IFNγ-IL8 Signaling-Induced Antibacterial Neutrophil States during Bacterial Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Single-cell atlas in the living fossil Yangtze sturgeon provides insight into the evolution of fish.Genome biology · 2025Article
- Single-cell RNA sequencing reveals hemocyte heterogeneity, differentiation trajectories, and viral tropism in shrimp (Journal of virology · 2025Article
- RNA-Seq Revealed the Effects of Cold Stress on Different Brain Regions ofAnimals : an open access journal from MDPI · 2025Article
- Temporal profiling of host transcriptome highlights time- and tissue-dependent Interferon pathway activation in NNV-infected European sea bass.Scientific reports · 2025Article
- Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass.BMC biology · 2025Article
- RNA-seq revealed the effects of heat stress on different brain regions ofFrontiers in physiology · 2025Article
- Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin FishViruses · 2024Article
- M1-type polarized macrophage contributes to brain damage through CXCR3.2/CXCL11 pathways after RGNNV infection in grouper.Virulence · 2024Article
- Decoding the fish genome opens a new era in important trait research and molecular breeding in China.Science China. Life sciences · 2024Review
- Nectin1 is a pivotal host factor involved in attachment and entry of red-spotted grouper nervous necrosis virus in the early stages of the viral life cycle.Journal of virology · 2024Article
- Slc43a2Science China. Life sciences · 2024Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral nervous necrosis (VNN) is an acute and serious fish disease caused by nervous necrosis virus (NNV) which has been reported massive mortality in more than fifty teleost species worldwide. VNN causes damage of necrosis and vacuolation to central nervous system (CNS) cells in fish. It is difficult to identify the specific type of cell targeted by NNV, and to decipher the host immune response because of the functional diversity and highly complex anatomical and cellular composition of the CNS. In this study, we found that the red spotted grouper NNV (RGNNV) mainly attacked the midbrain of orange-spotted grouper (Epinephelus coioides). We conducted single-cell RNA-seq analysis of the midbrain of healthy and RGNNV-infected fish and identified 35 transcriptionally distinct cell subtypes, including 28 neuronal and 7 non-neuronal cell types. An evaluation of the subpopulations of immune cells revealed that macrophages were enriched in RGNNV-infected fish, and the transcriptional profiles of macrophages indicated an acute cytokine and inflammatory response. Unsupervised pseudotime analysis of immune cells showed that microglia transformed into M1-type activated macrophages to produce cytokines to reduce the damage to nerve tissue caused by the virus. We also found that RGNNV targeted neuronal cell types was GLU1 and GLU3, and we found that the key genes and pathways by which causes cell cytoplasmic vacuoles and autophagy significant enrichment, this may be the major route viruses cause cell death. These data provided a comprehensive transcriptional perspective of the grouper midbrain and the basis for further research on how viruses infect the teleost CNS.
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