ArticleACS synthetic biology2021
Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles.
Article in ACS synthetic biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Bottom-up reconstitution of model membrane systems: a mini-review.Biochemical Society transactions · 2026Review
- Employing molecular beacons to assess in vitro transcription with single-molecule resolution.Scientific reports · 2026Article
- QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles.ACS applied materials & interfaces · 2026Article
- Image-based phenotypic sorting of synthetic cells.Science advances · 2026Article
- Quantification of cholesterol incorporation in giant unilamellar vesicles produced by a modified cDICE method.Soft matter · 2026Article
- A robust method for on-chip production and manipulation of lipid vesicles by inverted emulsion.Cell reports methods · 2026Article
- Tau-Driven Coordination of Microtubule-Actin Crosstalk in Cell-Sized Vesicles.bioRxiv : the preprint server for biology · 2025Article
- Strategy for Generating Giant Unilamellar Vesicles with Tunable Size Using the Modified cDICE Method.ACS synthetic biology · 2025Article
- Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Reconstituted systems for studying the architecture and dynamics of actin networks.The Biochemical journal · 2025Review
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Review
- Active membrane deformations of a minimal synthetic cell.Nature physics · 2025Article
- Article
- Nucleated synthetic cells with genetically driven intercompartment communication.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Building Synthetic Cells─From the Technology Infrastructure to Cellular Entities.ACS synthetic biology · 2024Review
- Hybrid Vesicles Enable Mechano-Responsive Hydrogel Degradation.Angewandte Chemie (International ed. in English) · 2023Article
- Engineering DNA-based cytoskeletons for synthetic cells.Interface focus · 2023Review
- Article
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10 authors.
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Abstract
Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in reconstitution experiments. They are also widely used in research on synthetic cells, as they provide a mechanically responsive reaction compartment that allows for controlled exchange of reactants with the environment. However, while many methods exist to encapsulate functional biomolecules in GUVs, there is no one-size-fits-all solution and reliable GUV fabrication still remains a major experimental hurdle in the field. Here, we show that defect-free GUVs containing complex biochemical systems can be generated by optimizing a double-emulsion method for GUV formation called continuous droplet interface crossing encapsulation (cDICE). By tightly controlling environmental conditions and tuning the lipid-in-oil dispersion, we show that it is possible to significantly improve the reproducibility of high-quality GUV formation as well as the encapsulation efficiency. We demonstrate efficient encapsulation for a range of biological systems including a minimal actin cytoskeleton, membrane-anchored DNA nanostructures, and a functional PURE (protein synthesis using recombinant elements) system. Our optimized cDICE method displays promising potential to become a standard method in biophysics and bottom-up synthetic biology.
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