Evidence map›Paper›PMID 34185215›Full record

ArticleBiotechnology letters2021

A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora.

Ye Li, Hui Feng, Lihua Jin, Xiulan Xin, Qipeng Yuan

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In one paragraph

Article in Biotechnology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ye Li *Department of Biotechnology, Beijing Polytechnic, No. 9, Liang Shuihe First Street, Yi Zhuang Economic & Technological Development Zone, Beijing, 100176, China.
Hui Feng *Department of Biotechnology, Beijing Polytechnic, No. 9, Liang Shuihe First Street, Yi Zhuang Economic & Technological Development Zone, Beijing, 100176, China.
Lihua JinDepartment of Biotechnology, Beijing Polytechnic, No. 9, Liang Shuihe First Street, Yi Zhuang Economic & Technological Development Zone, Beijing, 100176, China.
Xiulan XinDepartment of Biotechnology, Beijing Polytechnic, No. 9, Liang Shuihe First Street, Yi Zhuang Economic & Technological Development Zone, Beijing, 100176, China.
Qipeng YuanState Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, No. 15 East Road of North Third Ring, Chao Yang District, Beijing, 100029, China. yuanqp@mail.buct.edu.cn.ORCID http://orcid.org/0000-0003-3245-451X
Beijing Polytechnic · CNBeijing University of Chemical Technology · CN

Funding

Beijing Municipal Natural Science Foundation 2182019Excellent Talents of Organization Department of Beijing Municipal Committee 2020Z002-002-KWTGeneral Projects of Scientific Research Plan of Beijing Municipal Commission of Education KM201910858002
6 · The paper itself

Abstract

purposeThere are several studies on the use of RNA interference (RNAi) for gene function analysis in fungi. However, most studies on filamentous fungi are based on in vitro-transcribed or -synthesized small interfering RNA (siRNA), and only a few have reported the use of vector-based RNAi. Here we want to develop and evaluate a new vector-based RNAi method using the mouse U6 promoter to drive short hairpin RNA (shRNA) expression in the filamentous fungi.

methodsMolecular techniques were employed to develop and evaluate a new vector-based RNAi method using the mouse U6 promoter to drive short hairpin RNA (shRNA) expression in the filamentous fungus Blakeslea trispora.

resultsWe characterized the mouse U6 promoter and utilized it for the expression of shRNA in B. trispora. Using real-time polymerase chain reaction and western blotting analyses, we confirmed the decrease in the mRNA and protein expression of carRA, respectively, in cells transformed with the mouse U6 promoter-driven shRNA expression vector. This indicated that the shRNA was transcribed from the mouse U6 promoter and correctly processed into siRNA and that the mouse U6 promoter exhibited transcription ability in the filamentous fungi.

conclusionsThe results suggest that the mouse U6 promoter that drives the expression of shRNA vectors may serve as a novel tool for RNAi induction in filamentous fungi.

Indexed as

AnimalsFungal ProteinsGene Expression Regulation, FungalMiceMucoralesPlasmidsPromoter Regions, GeneticRNA InterferenceRNA, Small InterferingFungal ProteinsRNA, Small InterferingBlakeslea trisporacarRAFilamentous fungusMouse U6 promoterRNA interferenceshRNA

Identifiers

PMID34185215
OpenAlexW3173517035

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