ArticlePhysiological reports2021
Characterization of perfusion decellularized whole animal body, isolated organs, and multi-organ systems for tissue engineering applications.
Article in Physiological reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Current strategies on kidney regeneration using tissue engineering approaches: a systematic review.BMC nephrology · 2025Pooled it
- Comparative proteomic analysis of the composition of dECM and dECM-based inks compared to native tissues.iScience · 2026Article
- Decellularized Rat Lung Extracellular Matrix as an In Vitro Platform for Canine Yolk Sac-Derived Endothelial Precursor Cells for Pulmonary Endothelium Reconstruction Studies.Bioengineering (Basel, Switzerland) · 2026Article
- Development of Acellular Hepatic Scaffolds Through a Low-Cost Gravity-Assisted Perfusion Decellularization Method.Biomimetics (Basel, Switzerland) · 2025Article
- Research and application of thermosensitive Pickering emulsion with X-ray and ultrasound dual-modal imaging functions for intra-arterial embolization treatment.Journal of pharmaceutical analysis · 2025Article
- Application of tergitol in liver decellularization and bioink preparation based on the obtained extracellular matrix.Frontiers in bioengineering and biotechnology · 2025Article
- Efficient decellularization of human fetal kidneys through optimized SDS exposure.Scientific reports · 2024Article
- Extracellular matrix-derived materials for tissue engineering and regenerative medicine: A journey from isolation to characterization and application.Bioactive materials · 2024Review
- Article
- Article
- Effects of Different Perfusing Routes through The Portal Vein, Hepatic Vein, and Biliary Duct on Whole Rat Liver Decellularization.Cell journal · 2023Article
- Pushing the boundaries of innovation: the potential ofFrontiers in cardiovascular medicine · 2023Review
- Utility of perfusion decellularization to achieve biochemical and mechanically accurate whole animal and organ-specific tissue scaffolds.Physiological reports · 2021Article
- Innovative bioinks for 3D bioprinting: Exploring technological potential and regulatory challenges.Journal of tissue engineeringReview
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Authors and funding
12 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To expand the application of perfusion decellularization beyond isolated single organs, we used the native vasculature of adult and neonatal rats to systemically decellularize the organs of a whole animal in situ. Acellular scaffolds were generated from kidney, liver, lower limb, heart-lung system, and a whole animal body, demonstrating that perfusion decellularization technology is applicable to any perfusable tissue, independent of age. Biochemical and histological analyses demonstrated that organs and organ systems (heart-lung pair and lower limb) were successfully decellularized, retaining their extracellular matrix (ECM) structure and organ-specific composition, as evidenced by differences in organ-specific scaffold stiffness. Altogether, we demonstrated that organs, organ systems and whole animal bodies can be perfusion decellularized while retaining ECM components and biomechanics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.