ArticleScientific reports2021
Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Reference Gene Stability inInternational journal of molecular sciences · 2026Article
- Transcriptome-guided selection of stable reference genes for expression analysis in spinach.Scientific reports · 2024Article
- Genome-Wide Transcriptomic and Metabolomic Analyses Unveiling the Defence Mechanisms ofInternational journal of molecular sciences · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.PloS one · 2024Article
- Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis inPlants (Basel, Switzerland) · 2023Article
- Selection and Validation of the Most Suitable Reference Genes for Quantitative Real-Time PCR Normalization inPlants (Basel, Switzerland) · 2022Article
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5 authors.
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Abstract
Bromus sterilis is an annual weedy grass, causing high yield losses in winter cereals. Frequent use of herbicides had led to the evolution of herbicide resistance in this species. Mechanisms underlying herbicide resistance in B. sterilis must be uncovered because this problem is becoming a global threat. qRT-PCR and the next-generation sequencing technologies can elucidate the resistance mechanisms. Although qRT-PCR can calculate precise fold changes, its preciseness depends on the expression of reference genes. Regardless of stable expression in any given condition, no gene can act as a universal reference gene. Hence, it is necessary to identify the suitable reference gene for each species. To our knowledge, there are no reports on the suitable reference gene in any brome species so far. Thus, in this paper, the stability of eight genes was evaluated using qRT-PCR experiments followed by expression stability ranking via five most commonly used software for reference gene selection. Our findings suggest using a combination of 18S rRNA and ACCase to normalise the qRT-PCR data in B. sterilis. Besides, reference genes are also recommended for different experimental conditions. The present study outcomes will facilitate future molecular work in B. sterilis and other related grass species.
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