Evidence map›Paper›PMID 34183680›Full record

ArticleScientific data2021

Chromosomal-scale de novo genome assemblies of Cynomolgus Macaque and Common Marmoset.

Vasanthan Jayakumar, Osamu Nishimura, Mitsutaka Kadota, Naoki Hirose, Hiromi Sano, Yasuhiro Murakawa, Yumiko Yamamoto, Masataka Nakaya, Tomoyuki Tsukiyama, Yasunari Seita and 7 more

Abstract readDataset
In one paragraph

Article in Scientific data, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. A Complete Genome for the Common Marmoset.bioRxiv : the preprint server for biology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Vasanthan JayakumarDepartment of Biosciences and Informatics, Keio University, Yokohama, Kanagawa, 223-8522, Japan.
Osamu NishimuraLaboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, Minatojimaminami-machi 2-2-3, Kobe, Hyogo, 650-0047, Japan.
Mitsutaka KadotaLaboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, Minatojimaminami-machi 2-2-3, Kobe, Hyogo, 650-0047, Japan.
Naoki HiroseRIKEN Center for Integrative Medical Science Preventive Medicine and Applied Genomics Unit, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.ORCID 0000-0002-3358-0916
Hiromi SanoRIKEN Center for Integrative Medical Science Preventive Medicine and Applied Genomics Unit, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Yasuhiro MurakawaRIKEN Center for Integrative Medical Sciences RIKEN-IFOM Joint Laboratory for Cancer Genomics, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Yumiko YamamotoRIKEN Center for Integrative Medical Sciences Laboratory for Comprehensive Genomic Analysis, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Masataka NakayaDepartment of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, 520-2192, Japan.
Tomoyuki TsukiyamaDepartment of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, 520-2192, Japan.ORCID 0000-0002-2657-7719
Yasunari SeitaDepartment of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, 520-2192, Japan.
Shinichiro NakamuraDepartment of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, 520-2192, Japan.
Jun KawaiRIKEN Preventive Medicine and Diagnosis Innovation Program, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Erika SasakiCentral Institute for Experimental Animals, Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, 3-25-12, Tonomachi, Kawasaki-ku, Kawasaki, 210-0821, Japan.
Masatsugu EmaDepartment of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, 520-2192, Japan.ORCID 0000-0003-0645-6183
Shigehiro KurakuLaboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, Minatojimaminami-machi 2-2-3, Kobe, Hyogo, 650-0047, Japan.
Hideya KawajiRIKEN Center for Integrative Medical Science Preventive Medicine and Applied Genomics Unit, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan. hideya.kawaji@riken.jp.ORCID 0000-0002-0575-0308
Yasubumi SakakibaraDepartment of Biosciences and Informatics, Keio University, Yokohama, Kanagawa, 223-8522, Japan. yasu@bio.keio.ac.jp.ORCID 0000-0002-6932-3452

Funding

Japan Agency for Medical Research and Development (AMED) JP17kk0305008
6 · The paper itself

Abstract

Cynomolgus macaque (Macaca fascicularis) and common marmoset (Callithrix jacchus) have been widely used in human biomedical research. Long-standing primate genome assemblies used the human genome as a reference for ordering and orienting the assembled fragments into chromosomes. Here we performed de novo genome assembly of these two species without any human genome-based bias observed in the genome assemblies released earlier. We assembled PacBio long reads, and the resultant contigs were scaffolded with Hi-C data, which were further refined based on Hi-C contact maps and alternate de novo assemblies. The assemblies achieved scaffold N50 lengths of 149 Mb and 137 Mb for cynomolgus macaque and common marmoset, respectively. The high fidelity of our assembly is also ascertained by BAC-end concordance in common marmoset. Our assembly of cynomolgus macaque outperformed all the available assemblies of this species in terms of contiguity. The chromosome-scale genome assemblies produced in this study are valuable resources for non-human primate models and provide an important baseline in human biomedical research.

Indexed as

Contig MappingAnimalsCallithrixChromosomesGene OrderMacaca fascicularis

Identifiers

PMID34183680
PMCPMC8239027

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.