ArticleProceedings of the National Academy of Sciences of the United States of America2021
Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 28 citations in OpenAlex.
- Comprehensive metabolomic profiling of Angiostrongylus cantonensis across its life cycle reveals developmental and host-associated metabolic signatures.Infectious diseases of poverty · 2026Article
- An engineered bacterial symbiont maps micron-scale sugar gradients in the honeybee gut.PLoS biology · 2026Article
- Spatial biology using single-cell mass spectrometry imaging and integrated microscopy.Nature communications · 2025Article
- Mass Spectrometry Imaging: Revolutionizing Molecular Insights in Infectious Diseases Research.Pathogens (Basel, Switzerland) · 2025Review
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- Microbiome in cancer metastasis: biological insights and emerging spatial omics methods.Frontiers in cellular and infection microbiology · 2025Review
- pyM2aia: Python interface for mass spectrometry imaging with focus on deep learning.Bioinformatics (Oxford, England) · 2024Article
- Omics for deciphering oral microecology.International journal of oral science · 2024Review
- Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.Nature protocols · 2023Review
- Towards virtual histology with X-ray grating interferometry.Scientific reports · 2023Article
- Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging.PNAS nexus · 2023Article
- Coral holobiont research needs spatial analyses at the microbial scale.Environmental microbiology · 2023Article
- Review
- Taking a microscale look at symbiotic interactions-and why it matters.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues.Frontiers in chemistry · 2021Review
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our understanding of metabolic interactions between small symbiotic animals and bacteria or parasitic eukaryotes that reside within their bodies is extremely limited. This gap in knowledge originates from a methodological challenge, namely to connect histological changes in host tissues induced by beneficial and parasitic (micro)organisms to the underlying metabolites. We addressed this challenge and developed chemo-histo-tomography (CHEMHIST), a culture-independent approach to connect anatomic structure and metabolic function in millimeter-sized symbiotic animals. CHEMHIST combines chemical imaging of metabolites based on mass spectrometry imaging (MSI) and microanatomy-based micro-computed X-ray tomography (micro-CT) on the same animal. Both high-resolution MSI and micro-CT allowed us to correlate the distribution of metabolites to the same animal's three-dimensional (3D) histology down to submicrometer resolutions. Our protocol is compatible with tissue-specific DNA sequencing and fluorescence in situ hybridization for the taxonomic identification and localization of the associated micro(organisms). Building CHEMHIST upon in situ imaging, we sampled an earthworm from its natural habitat and created an interactive 3D model of its physical and chemical interactions with bacteria and parasitic nematodes in its tissues. Combining MSI and micro-CT, we present a methodological groundwork for connecting metabolic and anatomic phenotypes of small symbiotic animals that often represent keystone species for ecosystem functioning.
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