ArticleMalaria journal2021
Multiplex PCR assay for the identification of eight Anopheles species belonging to the Hyrcanus, Barbirostris and Lindesayi groups.
Article in Malaria journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Different larval habitats and insecticide resistance frequency of Anopheles sinensis in Ganghwagun, Incheon, South Korea.Scientific reports · 2025Article
- Genetic diversity and spatiotemporal population structure of Anopheles sinensis in the Republic of Korea based on the mitochondrial cytochrome c oxidase subunit I (COI) marker.Osong public health and research perspectives · 2025Article
- Population genetic structure analysis of Anopheles kleini in the Republic of Korea based on the mitochondrial COI gene.Malaria journal · 2025Article
- One-Step Multiplex Polymerase Chain Reaction Assay for the Detection of Major Disease-Transmitting Mosquito Vectors in India.The American journal of tropical medicine and hygiene · 2025Article
- Insecticide resistance mutations of Anopheles species in the Republic of Korea.PLoS neglected tropical diseases · 2025Article
- Using capillary electrophoresis to identify Anopheline species in routine sampling sites.Ecology and evolution · 2024Article
- Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea.Scientific reports · 2024Article
- Identification of breeding habitats and kdr mutations in Anopheles spp. in South Korea.Malaria journal · 2023Article
- Analysis of geometric morphometrics and molecular phylogeny for Anopheles species in the Republic of Korea.Scientific reports · 2023Article
- Phylogenetic Analysis of Some Species of theGenes · 2023Article
- PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras.Malaria journal · 2023Article
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Abstract
backgroundGenus Anopheles mosquitoes are the primary vectors of human malaria, which is a serious threat to public health worldwide. To reduce the spread of malaria and identify the malaria infection rates in mosquitoes, accurate species identification is needed. Malaria re-emerged in 1993 in the Republic of Korea (ROK), with numbers peaking in 2004 before decreasing to current levels. Eight Anopheles species (Anopheles sinensis, Anopheles pullus, Anopheles belenrae, Anopheles lesteri, Anopheles kleini, Anopheles sineroides, Anopheles koreicus, Anopheles lindesayi) are distributed throughout Korea. Members of the Anopheles Hyrcanus group currently cannot be identified morphologically. The other species of Anopheles can be identified morphologically, except when specimens are damaged in traps. The purpose of this study was to develop a rapid and accurate method for simultaneous molecular identification of the eight Anopheles species present in the ROK.
methodsAnopheles spp. used in this study were collected near/in the demilitarized zone in ROK, where most malaria cases are reported. DNA from 165 of the Anopheles specimens was used to develop a multiplex PCR assay. The internal transcribed spacer 2 (ITS2) region of each species was sequenced and analysed for molecular identification.
resultsDNA from a total of 165 Anopheles specimens was identified to species using a multiplex diagnostic system. These included: 20 An. sinensis, 21 An. koreicus, 17 An. lindesayi, 25 An. kleini, 11 An. lesteri, 22 An. sineroides, 23 An. belenrae, and 26 An. pullus. Each species was clearly distinguished by electrophoresis as follows: 1,112 bp for An. sinensis; 925 bp for An. koreicus; 650 bp for An. lindesayi; 527 bp for An. kleini; 436 bp for An. lesteri; 315 bp for An. sineroides; 260 bp for An. belenrae; and, 157 bp for An. pullus.
conclusionA multiplex PCR assay was developed to identify Anopheles spp. distributed in ROK. This method can be used to accurately identify Anopheles species that are difficult to identify morphologically to determine species distributions and malaria infection rates.
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