ArticleBMC microbiology2021
A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Microfluidic pretreatment devices for milk safety at the point of care.Mikrochimica acta · 2026Review
- Paper-Based Microfluidic Chips for At-Home Point-of-Care Nucleic Acid Testing: Applications and Challenges.Diagnostics (Basel, Switzerland) · 2026Review
- Article
- LAMP using distance based paper device for quantitative detection of ESBL producing Escherichia coli in UTI patients.Scientific reports · 2025Article
- Lab-on-a-Chip Devices for Nucleic Acid Analysis in Food Safety.Micromachines · 2024Review
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Advancements of paper-based sensors for antibiotic-resistant bacterial species identification.Npj biosensing · 2024Review
- Rapid detection methods for foodborne pathogens based on nucleic acid amplification: Recent advances, remaining challenges, and possible opportunities.Food chemistry. Molecular sciences · 2023Review
- Validation of quantitative loop-mediated isothermal amplification assay using a fluorescent distance-based paper device for detection of Escherichia coli in urine.Scientific reports · 2023Article
- Review
- Promise and perils of paper-based point-of-care nucleic acid detection for endemic and pandemic pathogens.Lab on a chip · 2023Review
- Antibiotic Resistance and Epidemiology ofInfection and drug resistance · 2023Article
- Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems.Frontiers in bioengineering and biotechnology · 2023Review
- Paper-based sensors for bacteria detection.Nature reviews bioengineering · 2023Review
- A portable system for economical nucleic acid amplification testing.Frontiers in bioengineering and biotechnology · 2023Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- MINI: A high-throughput point-of-care device for performing hundreds of nucleic acid tests per day.Biosensors & bioelectronics · 2022Article
- Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip.BMC microbiology · 2022Article
- Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip.Mikrochimica acta · 2022Article
- Detection of UnamplifiedBiosensors · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrofluidic chip detection technology is considered a potent tool for many bioanalytic applications. Rapid detection of foodborne pathogens in the early stages is imperative to prevent the outbreak of foodborne diseases, known as a severe threat to human health. Conventional bacterial culture methods for detecting foodborne pathogens are time-consuming, laborious, and lacking in pathogen diagnosis. To overcome this problem, we have created an embedded paper-based microchip based on isothermal loop amplification (LAMP), which can rapidly and sensitively detect foodborne pathogens.
resultsWe embed paper impregnated with LAMP reagent and specific primers in multiple reaction chambers of the microchip. The solution containing the target pathogen was injected into the center chamber and uniformly distributed into the reaction chamber by centrifugal force. The purified DNA of Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, and Vibrio parahaemolyticus has been successfully amplified and directly detected on the microchip. The E. coli O157:H7 DNA was identified as low as 0.0134 ng μL
conclusionThe paper embedded LAMP microchip offers inexpensive, user-friendly, and highly selective pathogen detection capabilities. It is expected to have great potential as a quick, efficient, and cost-effective solution for future foodborne pathogen detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.