ArticlemBio2021
ATP Drives Efficient Terpene Biosynthesis in Marine Thraustochytrids.
Article in mBio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Bioactivities and biosynthesis of monoterpene-based biopesticides: current state and perspectives.Microbial cell factories · 2026Review
- Acetate prevents pistil dysfunction in rice under heat stress by inducing methyl jasmonate and quercetin synthesis.Journal of advanced research · 2025Article
- Production of squalene and fatty acids by Thraustochytrium sp. RT2316-16: effects of dissolved oxygen and the medium composition.Bioresources and bioprocessing · 2025Article
- Salinity Modulates Carbon Flux to Promote Squalene and PUFA Biosynthesis in the Marine ProtistMarine drugs · 2025Article
- Multiple factors based adaptive laboratory evolution strategy enhances DHA production in Aurantiochytrium sp. PKU#Mn16 by rewiring key metabolic pathways.Microbial cell factories · 2025Article
- Bioprocess conditions and regulation factors to optimize squalene production in thraustochytrids.World journal of microbiology & biotechnology · 2023Review
- Optimal NaCl Medium Enhances Squalene Accumulation inFrontiers in microbiology · 2022Article
- An emerging simple and effective approach to increase the productivity of thraustochytrids microbial lipids by regulating glycolysis process and triacylglycerols' decomposition.Biotechnology for biofuels · 2021Article
- Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content ofMarine drugs · 2021Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding carbon flux controlling mechanisms in a tangled metabolic network is an essential question of cell metabolism. Secondary metabolism, such as terpene biosynthesis, has evolved with low carbon flux due to inherent pathway constraints. Thraustochytrids are a group of heterotrophic marine unicellular protists and can accumulate terpenoids under the high-salt conditions in their natural environment. However, the mechanism behind terpene accumulation is not well understood. Here, we show that terpene biosynthesis in Thraustochytrium sp. ATCC 26185 is constrained by local thermodynamics in the mevalonate pathway. Thermodynamic analysis reveals metabolite limitation in the nondecarboxylative Claisen condensation of acetyl-coenzyme A (CoA) to the acetoacetyl-CoA step, catalyzed by the acetyl-CoA acetyltransferase (ACAT). Through a sodium-elicited mechanism, higher respiration leads to increased ATP investment into the mevalonate pathway, providing a strong thermodynamic driving force for enhanced terpene biosynthesis. Proteomic and metabolomic analyses further show that the increased ATP demands are fulfilled by shifting energy generation from carbohydrate to lipid oxidation. This study demonstrates a unique strategy in nature that uses ATP to drive a low-flux metabolic pathway, providing an alternative solution for efficient terpene metabolic engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.