ArticleJournal of cellular and molecular medicine2021
Evaluation of five column-based isolation kits and their ability to extract miRNA from human milk.
Article in Journal of cellular and molecular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- A selective enrichment and specific probe terminal mediated strategy for highly sensitive detection of microRNAs.Nature communications · 2026Article
- Characterizing Human Milk RNA Degradation Over Time to Optimize Storage of Human Milk for RNA Quality.Journal of mammary gland biology and neoplasia · 2026Article
- The yields of nucleic acids and proteins extracted from various murine tissue types.Acta biochimica Polonica · 2026Article
- ncRNAs in breast milk of mothers with SLE: potential implications for neonatal immune development.Frontiers in immunology · 2026Review
- Developing Up-Scale Allogeneic Chondrocyte Therapies Using Juvenile Donor Cartilage.International journal of molecular sciences · 2025Article
- Transcriptomic profiling of milk fat globules in cows with different β-casein genotypes.Scientific reports · 2025Article
- Preservation of milk in liquid nitrogen during sample collection does not affect the RNA quality for RNA-seq analysis.BMC genomics · 2025Article
- Role of microRNAs in Immune Regulation with Translational and Clinical Applications.International journal of molecular sciences · 2024Review
- Article
- A comparative study on the expression of myogenic genes, and their effects on performance and meat quality in broiler chicken strains.Veterinary research forum : an international quarterly journal · 2024Article
- Occurrence, Role, and Challenges of MicroRNA in Human Breast Milk: A Scoping Review.Biomedicines · 2023Article
- Importance of Extracellular Vesicle Derived RNAs as Critical Colorectal Cancer Biomarkers.ACS bio & med chem Au · 2022Review
- Evaluation of five column-based isolation kits and their ability to extract miRNA from human milk.Journal of cellular and molecular medicine · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNA can be found in various body fluids, including breast milk. MicroRNA may be transferred from mother to infant via breast milk and potentially regulate the development of the infant's immune system on a post-transcriptional level. This study aimed to determine the microRNA extraction efficiency of five RNA extraction kits from human skim milk samples. Their efficiency was determined by comparing microRNA concentrations, total RNA yield and purity. Furthermore, hsa-miR-148a-3p expression and the recovery of an exogenous control, cel-miR-39-3p, were quantified using qPCR. Each kit extracted different amounts of microRNA and total RNA, with one kit tending to isolate the highest amount of both RNA species. Based on these results, the extraction kit ReliaPrep™ miRNA Cell and Tissue Miniprep System from Promega was found to be the most appropriate kit for microRNA extraction from human skim milk. Moreover, further research is needed to establish a standardized protocol for microRNA extraction from breast milk.
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