ArticleThe Journal of cell biology1988
Fluorescent microtubules break up under illumination.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed.
- Multi-camera Simultaneous Total Internal Reflection and Interference Reflection Microscopy.bioRxiv : the preprint server for biology · 2024Article
- Laser-induced microinjury of the corneal basal epithelium and imaging of resident macrophage responses in a live, whole-eye preparation.Frontiers in immunology · 2023Article
- The rate of microtubule breaking increases exponentially with curvature.Scientific reports · 2022Article
- In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Reflection Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- α-tubulin tail modifications regulate microtubule stability through selective effector recruitment, not changes in intrinsic polymer dynamics.Developmental cell · 2021Article
- Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.Journal of the American Chemical Society · 2020Article
- In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Focusing super resolution on the cytoskeleton.F1000Research · 2016Review
- Ultra-stable organic fluorophores for single-molecule research.Chemical Society reviews · 2014Review
- An improved optical tweezers assay for measuring the force generation of single kinesin molecules.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Intermittent depolymerization of actin filaments is caused by photo-induced dimerization of actin protomers.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Cargo loading onto kinesin powered molecular shuttles.Journal of visualized experiments : JoVE · 2010Article
- In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces.Methods in cell biology · 2010Review
- Chromophore-assisted laser inactivation in cell biology.Trends in cell biology · 2008Review
- Mechanism and dynamics of breakage of fluorescent microtubules.Biophysical journal · 2006Article
- Mobility of taxol in microtubule bundles.Biophysical journal · 2003Article
- Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling.The Journal of cell biology · 1997Article
- Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro.Molecular biology of the cell · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously been used in this and other laboratories. Our results show that all four analogues assemble into microtubules which break up when exposed to light of the wavelengths that excite fluorescence. This sensitivity places severe constraints on the use of these analogues in studies of microtubule dynamics.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.