ArticleThe Journal of cell biology1988
Posttranslational incorporation of contractile proteins into myofibrils in a cell-free system.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Dynamics of Z-band based proteins in developing skeletal muscle cells.Cell motility and the cytoskeleton · 2005Article
- A calcium signaling cascade essential for myosin thick filament assembly in Xenopus myocytes.The Journal of cell biology · 1998Article
- Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly.The Journal of cell biology · 1995Article
- Antisense suppression of skeletal muscle myosin light chain-1 biosynthesis impairs myofibrillogenesis in cultured myotubes.Journal of muscle research and cell motility · 1995Article
- Mutant actin: a dead end?Journal of muscle research and cell motility · 1992Review
- Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development.The Journal of cell biology · 1991Article
- Cell-free incorporation of newly synthesized myosin subunits into thick myofilaments.Journal of muscle research and cell motility · 1991Article
- Myosin mRNA accumulation and myofibrillogenesis at the myotendinous junction of stretched muscle fibers.The Journal of cell biology · 1990Article
- Subcellular localization of newly incorporated myosin in rabbit fast skeletal muscle undergoing stimulation-induced type transformation.Journal of muscle research and cell motility · 1990Article
- Isolation and characterization of a cDNA clone encoding avian skeletal muscle C-protein: an intracellular member of the immunoglobulin superfamily.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Size and charge heterogeneity of C-protein isoforms in avian skeletal muscle. Expression of six different isoforms in chicken muscle.Journal of muscle research and cell motility · 1989Article
- Genetic analysis of myosin assembly in Caenorhabditis elegans.Molecular neurobiologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The incorporation of newly synthesized protein into myofibrils has been examined in a cell-free system. Myofibrils were added to a reticulocyte lysate after the in vitro translation of muscle-specific poly(A)+RNA. Only a small number of the many synthesized proteins were found to associate with the exogenously added myofibrils. These proteins were all identified as sarcomeric components and had subunit mobilities (Mr) of 200, 140, 95, 86, 43, 38, 35, 25, 23, 20, and 18 kD. The association was rapid (t1/2 less than 15 min) and, for most of the proteins, relatively temperature insensitive. Except for a 43-kD polypeptide, tentatively identified as beta-actin, none of the proteins encoded by brain poly(A)+RNA associated with the myofibrils. When filaments made from purified myosin or actin were used as the "capture" substrates, only thick or thin filament proteins, respectively, were incorporated. Incorporation was substantially reduced when cross-linked myosin filaments were used. These results are compatible with a model in which proteins of the sarcomere are in kinetic equilibrium with homologous proteins in a soluble pool.
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Registered trials
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