Evidence map›Paper›PMID 34177328›Full record

ArticlePlant biotechnology (Tokyo, Japan)2021

Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc.

Ken-Ichiro Taoka, Zenpei Shimatani, Koji Yamaguchi, Mana Ogawa, Hiromi Saitoh, Yoichi Ikeda, Hiroko Akashi, Rie Terada, Tsutomu Kawasaki, Hiroyuki Tsuji

Open access · diamondAbstract read
In one paragraph

Article in Plant biotechnology (Tokyo, Japan), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. A NanoLuc-Based Transactivation Assay in Plants.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Ken-Ichiro TaokaKihara Institute for Biological Research, Yokohama City University, Kanagawa 244-0813, Japan.
Zenpei ShimataniGraduate School of Science, Technology and Innovation, Kobe University, Hyogo 657-8501, Japan.
Koji YamaguchiDepartment of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara 631-8505, Japan.
Mana OgawaGraduate School of Agriculture, Meijo University, Aichi 468-8502, Japan.
Hiromi SaitohGraduate School of Agriculture, Meijo University, Aichi 468-8502, Japan.
Yoichi IkedaGraduate School of Agriculture, Meijo University, Aichi 468-8502, Japan.
Hiroko AkashiKihara Institute for Biological Research, Yokohama City University, Kanagawa 244-0813, Japan.
Rie TeradaGraduate School of Agriculture, Meijo University, Aichi 468-8502, Japan.
Tsutomu KawasakiDepartment of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara 631-8505, Japan.
Hiroyuki TsujiKihara Institute for Biological Research, Yokohama City University, Kanagawa 244-0813, Japan.
Meijo University · JPKindai University · JPYokohama City University · JPKihara Institute for Biological Research · JPKobe University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (

Indexed as

florigenknock-inNanoLucprotein-protein interactionrice

Identifiers

PMID34177328
PMCPMC8215459
OpenAlexW3134944535

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.