ArticleBiotechnology for biofuels2021
Engineering of Yarrowia lipolytica transporters for high-efficient production of biobased succinic acid from glucose.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production inSynthetic and systems biotechnology · 2026Article
- Development of a CRISPR/Cas12a genome editing toolbox inSynthetic and systems biotechnology · 2026Article
- Microbial Bio-refineries: Engineering Microbes for the Production of Versatile Short-Chain Organic Acids.Indian journal of microbiology · 2025Review
- Synergistic effect of transporter and pathway engineering on the key performance indicators of erythritol synthesis by the yeastApplied and environmental microbiology · 2025Article
- EngineeringJournal of microbiology and biotechnology · 2025Article
- Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.FEMS yeast research · 2025Review
- Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry.Biotechnology for biofuels and bioproducts · 2024Review
- Strategies to increase the robustness of microbial cell factories.Advanced biotechnology · 2024Review
- Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica.Nature communications · 2023Article
- An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.Nature communications · 2023Article
- Fungal carboxylate transporters: recent manipulations and applications.Applied microbiology and biotechnology · 2023Review
- A review of synthetic biology tools in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- New roles for Yarrowia lipolytica in molecules synthesis and biocontrol.Applied microbiology and biotechnology · 2022Review
- Potential one-step strategy for PET degradation and PHB biosynthesis through co-cultivation of two engineered microorganisms.Engineering microbiology · 2021Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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Abstract
backgroundSuccinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology industry. Yarrowia lipolytica has a strong tricarboxylic acid cycle and tolerates low pH conditions, thus making it a potential platform for SA production. However, its SA titers in glucose media remain low.
resultsIn this study, we screened mitochondrial carriers and C4-dicarboxylic acid transporters to enhance SA secretion in Y. lipolytica. PGC62-SYF-Mae strain with efficient growth and SA production was constructed by optimizing SA biosynthetic pathways and expressing the transporter SpMae1. In fed-batch fermentation, this strain produced 101.4 g/L SA with a productivity of 0.70 g/L/h and a yield of 0.37 g/g glucose, which is the highest SA titer achieved using yeast, with glucose as the sole carbon resource.
conclusionOur results indicated that transporter engineering is a powerful strategy to achieve the efficient secretion of SA in Y. lipolytica, which will promote the industrial production of bio-based SA.
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