ArticleParasites & vectors2021
A conserved female-specific larval requirement for MtnB function facilitates sex separation in multiple species of disease vector mosquitoes.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Targeting the Highly Invasive Malaria Vector Anopheles stephensi using Yeast RNAi Pesticides.Research square · 2026Article
- Advances in male sex separation for the support of mosquito control programs.Frontiers in insect science · 2026Review
- Requirements for market entry of gene drive-modified mosquitoes for control of vector-borne diseases: analogies to other biologic and biotechnology products.Frontiers in bioengineering and biotechnology · 2023Review
- Selective targeting of biting females to control mosquito-borne infectious diseases.Trends in parasitology · 2022Review
- VectorBase.org updates: bioinformatic resources for invertebrate vectors of human pathogens and related organisms.Current opinion in insect science · 2022Review
- A Conserved Female-Specific Requirement for thePathogens (Basel, Switzerland) · 2022Article
- A Broad-Based Mosquito Yeast Interfering RNA Pesticide TargetingPathogens (Basel, Switzerland) · 2021Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundClusters of sex-specific loci are predicted to shape the boundaries of the M/m sex-determination locus of the dengue vector mosquito Aedes aegypti, but the identities of these genes are not known. Identification and characterization of these loci could promote a better understanding of mosquito sex chromosome evolution and lead to the elucidation of new strategies for male mosquito sex separation, a requirement for several emerging mosquito population control strategies that are dependent on the mass rearing and release of male mosquitoes. This investigation revealed that the methylthioribulose-1-phosphate dehydratase (MtnB) gene, which resides adjacent to the M/m locus and encodes an evolutionarily conserved component of the methionine salvage pathway, is required for survival of female larvae.
resultsLarval consumption of Saccharomyces cerevisiae (yeast) strains engineered to express interfering RNA corresponding to MtnB resulted in target gene silencing and significant female death, yet had no impact on A. aegypti male survival or fitness. Integration of the yeast larvicides into mass culturing protocols permitted scaled production of fit adult male mosquitoes. Moreover, silencing MtnB orthologs in Aedes albopictus, Anopheles gambiae, and Culex quinquefasciatus revealed a conserved female-specific larval requirement for MtnB among different species of mosquitoes.
conclusionsThe results of this investigation, which may have important implications for the study of mosquito sex chromosome evolution, indicate that silencing MtnB can facilitate sex separation in multiple species of disease vector insects.
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