ArticleAging cell2021
Single-cell RNA-Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation.
Article in Aging cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- TSG FromFood science & nutrition · 2026Article
- Dissecting the transcriptomic signature of porcine oocyte during primordial follicle formation from single cell resolution.Journal of ovarian research · 2026Article
- Gene regulatory networks orchestrating oocyte fate bifurcation in primordial follicles revealed by single-cell transcriptomics.Communications biology · 2026Article
- Identification of biomarkers for Laryngeal squamous cell carcinoma through Mendelian randomization and integrated bioinformatics analysis.Discover oncology · 2025Article
- Loss of FUT8 impairs embryonic development by reducing cAMP production in granulosa cells.Journal of assisted reproduction and genetics · 2025Article
- Decoding the Tumor Microenvironment of Myoepithelial Cells in Triple-Negative Breast Cancer Through Single-Cell and Transcriptomic Sequencing and Establishing a Prognostic Model Based on Key Myoepithelial Cell Genes.International journal of genomics · 2025Article
- HFM1 is essential for the germ cell intercellular bridge transport in primordial follicle formation in mice.Cellular and molecular life sciences : CMLS · 2024Article
- FOSMolecular therapy. Oncology · 2024Article
- Communication between alveolar macrophages and fibroblasts via the TNFSF12-TNFRSF12A pathway promotes pulmonary fibrosis in severe COVID-19 patients.Journal of translational medicine · 2024Article
- Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators.Nature cell biology · 2024Article
- Mesenchymal stem cells promote ovarian reconstruction in mice.Stem cell research & therapy · 2024Article
- Single-Cell Transcriptome Analysis Reveals Development-Specific Networks at Distinct Synchronized Antral Follicle Sizes in Sheep Oocytes.International journal of molecular sciences · 2024Article
- LSM14B controls oocyte mRNA storage and stability to ensure female fertility.Cellular and molecular life sciences : CMLS · 2023Article
- Single-cell RNA-seq identified novel genes involved in primordial follicle formation.Frontiers in endocrinology · 2023Article
- The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate.Cell & bioscience · 2022Article
- Effects and potential mechanism of CaInternational journal of molecular medicine · 2022Article
- FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination.Clinical and translational medicine · 2022Article
- Impaired primordial follicle assembly in offspring ovaries from zearalenone-exposed mothers involves reduced mitochondrial activity and altered epigenetics in oocytes.Cellular and molecular life sciences : CMLS · 2022Article
- Editorial: Reproductive genomics.Frontiers in genetics · 2022Article
- Single-cell RNA-Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation.Aging cell · 2021Article
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of primordial follicles in mammals represents one of the most critical processes in ovarian biology. It directly affects the number of oocytes available to a female throughout her reproductive life. Premature depletion of primordial follicles contributes to the ovarian pathology primary ovarian insufficiency (POI). To delineate the developmental trajectory and regulatory mechanisms of oocytes during the process, we performed RNA-seq on single germ cells from newborn (P0.5) ovaries. Three cell clusters were classified which corresponded to three cell states (germ cell cyst, cyst breakdown, and follicle) in the newborn ovary. By Monocle analysis, a uniform trajectory of oocyte development was built with a series of genes showed dynamic changes along the pseudo-timeline. Gene Ontology term enrichment revealed a significant decrease in meiosis-related genes and a dramatic increase in oocyte-specific genes which marked the transition from a germ cell to a functional oocyte. We then established a network of regulons by using single-cell regulatory network inference and clustering (SCENIC) algorithm and identified possible candidate transcription factors that may maintain transcription programs during follicle formation. Following functional studies further revealed the differential regulation of the identified regulon Id2 and its family member Id1, on the establishment of primordial follicle pool by using siRNA knockdown and genetic modified mouse models. In summary, our study systematically reconstructed molecular cascades in oocytes and identified a series of genes and molecular pathways in follicle formation and development.
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