ArticleFEMS microbiology letters2021
Heterologous expression of equol biosynthesis genes from Adlercreutzia equolifaciens.
Article in FEMS microbiology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Intestinal Microbiomics and Liver Metabolomics Insights into the Improvement Effects of Lactiplantibacillus plantarum FJG2627 on Lipopolysaccharide-Induced Acute Liver Injury in Mice.Probiotics and antimicrobial proteins · 2026Article
- How Gut Microbes Transform Dietary Phytonutrients: Enzymatic Pathways and Human Metabolite Exposure.Molecules (Basel, Switzerland) · 2026Review
- Intestinal Microbiota of Older Japanese Females Adhering to a Traditional Japanese Brown Rice-Based Diet Pattern.Nutrients · 2026Article
- In Vivo and In Vitro Mechanisms of Equol Synthesis and Key Influencing Factors: A Critical Review.Nutrients · 2025Review
- Article
- Association between microbiome and the development of adverse posttraumatic neuropsychiatric sequelae after traumatic stress exposure.Translational psychiatry · 2023Article
- Advances in the Metabolic Mechanism and Functional Characteristics of Equol.Foods (Basel, Switzerland) · 2023Review
- Cross-sectional association of equol producing status with aortic calcification in Japanese men aged 40-79 years.Scientific reports · 2022Article
- Gut Microbiota-Assisted Synthesis, Cellular Interactions and Synergistic Perspectives of Equol as a Potent Anticancer Isoflavone.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Taxonomic distribution and evolutionary analysis of the equol biosynthesis gene cluster.BMC genomics · 2022Article
- Review
- Functional recovery outcomes following acute stroke is associated with abundance of gut microbiota related to inflammation, butyrate and secondary bile acid.Frontiers in rehabilitation sciences · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Equol is the isoflavone-derived metabolite with the greatest estrogenic and antioxidant activity. It is produced from daidzein by fastidious and oxygen-susceptible intestinal bacteria, which hinders their use at an industrial scale. Therefore, expressing the equol production machinery into easily-cultivable hosts would expedite the heterologous production of this compound. In this work, four genes (racemase, tdr, ddr and dzr) coding for key enzymes involved in equol production in Adlercreutzia equolifaciens DSM19450T were synthesized and cloned in a pUC-derived vector (pUC57-equol) that was introduced in Escherichia coli. Recombinant clones of E. coli produced equol in cultures supplemented with daidzein (equol precursor) and dihydrodaidzein (intermediate compound). To check whether equol genes were expressed in Gram-positive bacteria, the pUC57-equol construct was cloned into the low-copy-number vector pIL252, and the new construct (pIL252-pUC57-equol) introduced into model strains of Lacticaseibacillus casei and Lactococcus lactis. L. casei clones carrying pIL252-pUC57-equol produced a small amount of equol from dihydrodaidzein but not from daidzein, while L. lactis recombinant clones produced no equol from either of the substrates. This is the first time that A. equolifaciens equol genes have been cloned and expressed in heterologous hosts. E. coli clones harboring pUC57-equol could be used for biotechnological production of equol.
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