ReviewTrends in parasitology2021
Single-cell genome sequencing of protozoan parasites.
Review in Trends in parasitology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Next-generation sequencing in tick-borne apicomplexan parasites: approaches for Theileria and Babesia, a systematic review.Tropical animal health and production · 2026Pooled it
- Molecular surveillance of multiplicity of infection, haplotype frequencies, and prevalence in infectious diseases.PLoS computational biology · 2026Article
- Sustained production and purification ofParasitology · 2025Article
- Article
- Beyond the first case of Chagas disease: the Berenice strain as a model for understanding long-term Trypanosoma cruzi infection.Memorias do Instituto Oswaldo Cruz · 2025Review
- Review of MrsFreqPhase methods: methods designed to estimate statistically malaria parasite multiplicity of infection, relatedness, frequency and phase.Malaria journal · 2024Review
- Microbial Diversity and Open Questions about the Deep Tree of Life.Genome biology and evolution · 2024Article
- Measurably recombining malaria parasites.Trends in parasitology · 2023Review
- Systems biology of malaria explored with nonhuman primates.Malaria journal · 2022Review
- A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David's deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China.BMC veterinary research · 2022Article
- Optimizing bulk segregant analysis of drug resistance usingiScience · 2022Article
- Parasite OMICS, the grand challenges ahead.Frontiers in parasitology · 2022Article
- Evolutionary genetics of malaria.Frontiers in genetics · 2022Article
- A maximum-likelihood method to estimate haplotype frequencies and prevalence alongside multiplicity of infection from SNP data.Frontiers in epidemiology · 2022Article
- Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?Pathogens (Basel, Switzerland) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Despite considerable genetic variation within hosts, most parasite genome sequencing studies focus on bulk samples composed of millions of cells. Analysis of bulk samples is biased toward the dominant genotype, concealing cell-to-cell variation and rare variants. To tackle this, single-cell sequencing approaches have been developed and tailored to specific host-parasite systems. These are allowing the genetic diversity and kinship in complex parasite populations to be deciphered and for de novo genetic variation to be captured. Here, we outline the methodologies being used for single-cell sequencing of parasitic protozoans, such as Plasmodium and Leishmania spp., and how these tools are being applied to understand parasite biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.