Evidence map›Paper›PMID 34171821›Full record

ArticleBiosensors & bioelectronics2021

Point-of-care CRISPR-Cas-assisted SARS-CoV-2 detection in an automated and portable droplet magnetofluidic device.

Fan-En Chen, Pei-Wei Lee, Alexander Y Trick, Joon Soo Park, Liben Chen, Kushagra Shah, Heba Mostafa, Karen C Carroll, Kuangwen Hsieh, Tza-Huei Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed, 3 pooled it
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 3 syntheses or guidelines pooled it, 101 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Fan-En ChenDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Pei-Wei LeeDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Alexander Y TrickDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Joon Soo ParkDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Liben ChenDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Kushagra ShahDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Heba MostafaDepartment of Pathology, Johns Hopkins University, School of Medicine, Baltimore, MD, 21287, USA.
Karen C CarrollDepartment of Pathology, Johns Hopkins University, School of Medicine, Baltimore, MD, 21287, USA.
Kuangwen HsiehDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA. Electronic address: khsieh4@jhu.edu.
Tza-Huei WangDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 21218, USA. Electronic address: thwang@jhu.edu.
Johns Hopkins University · US

Funding

Vaccine Response and Immunotherapeutics SWGP30AI094189 · NIAID · JOHNS HOPKINS UNIVERSITY · PI Richard E. Chaisson · 2012 to 2026
$67.0M
TrainingU54EB007958 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI Yukari C Manabe · 2007 to 2026
$34.6M
Technology development for point-of-care detection and antimicrobial susceptibility testing of Neisseria gonorrhoeaeR01AI138978 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF · 2018 to 2021
$5.1M
Streamlined 15-min HIV Viral Load Self-Testing Using Finger-Stick BloodR61AI154628 · NIAID · JOHNS HOPKINS UNIVERSITY · PI HSIEH, YU-HSIANG, MANABE, YUKARI C · 2020 to 2022
$1.4M
NIAID NIH HHS P30 AI094189NIAID NIH HHS R01 AI138978NIAID NIH HHS R61 AI154628NIBIB NIH HHS U54 EB007958
6 · The paper itself

Abstract

In the fight against COVID-19, there remains an unmet need for point-of-care (POC) diagnostic testing tools that can rapidly and sensitively detect the causative SARS-CoV-2 virus to control disease transmission and improve patient management. Emerging CRISPR-Cas-assisted SARS-CoV-2 detection assays are viewed as transformative solutions for POC diagnostic testing, but their lack of streamlined sample preparation and full integration within an automated and portable device hamper their potential for POC use. We report herein POC-CRISPR - a single-step CRISPR-Cas-assisted assay that incoporates sample preparation with minimal manual operation via facile magnetic-based nucleic acid concentration and transport. Moreover, POC-CRISPR has been adapted into a compact thermoplastic cartridge within a palm-sized yet fully-integrated and automated device. During analytical evaluation, POC-CRISPR was able detect 1 genome equivalent/μL SARS-CoV-2 RNA from a sample volume of 100 μL in < 30 min. When evaluated with 27 unprocessed clinical nasopharyngeal swab eluates that were pre-typed by standard RT-qPCR (C

Indexed as

Biosensing TechniquesCOVID-19Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsHumansPoint-of-Care SystemsRNA, ViralSARS-CoV-2Sensitivity and SpecificityRNA, ViralCRISPRPoint-of-CareSensorsViruses

Identifiers

PMID34171821
PMCPMC8170879
OpenAlexW3166186461

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.