Evidence map›Paper›PMID 34170616›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2021

Ultrasensitive Multiparameter Phenotyping of Rare Cells Using an Integrated Digital-Molecular-Counting Microfluidic Well Plate.

Shiuan-Haur Su, Yujing Song, Michael W Newstead, Tao Cai, MengXi Wu, Andrew Stephens, Benjamin H Singer, Katsuo Kurabayashi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Shiuan-Haur SuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-8956-3533
Yujing SongDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0003-1448-3069
Michael W NewsteadDepartment of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-2512-6377
Tao CaiDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-3391-9289
MengXi WuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0003-0086-5349
Andrew StephensDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0003-4890-7878
Benjamin H SingerDepartment of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-4721-6920
Katsuo KurabayashiDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-9613-3590
University of Michigan · US

Funding

Enhancement of Brain Dysfunction in Sepsis Survivors by Alzheimer’s Disease NeuropathologyR01AG074968 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Benjamin Herschel Singer · 2022 to 2026
$3.3M
β-Amyloid Potentiates Neuroinflammation and Cognitive Decline After Sepsis SurvivalK08NS101054 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SINGER, BENJAMIN HERSCHEL · 2017 to 2021
$1.0M
NIA NIH HHS R01 AG074968NINDS NIH HHS K08 NS101054
6 · The paper itself

Abstract

Integrated microfluidic cellular phenotyping platforms provide a promising means of studying a variety of inflammatory diseases mediated by cell-secreted cytokines. However, immunosensors integrated in previous microfluidic platforms lack the sensitivity to detect small signals in the cellular secretion of proinflammatory cytokines with high precision. This limitation prohibits researchers from studying cells secreting cytokines at low abundance or existing at a small population. Herein, the authors present an integrated platform named the "digital Phenoplate (dPP)," which integrates digital immunosensors into a microfluidic chip with on-chip cell assay chambers, and demonstrates ultrasensitive cellular cytokine secretory profile measurement. The integrated sensors yield a limit of detection as small as 0.25 pg mL

Indexed as

Biosensing TechniquesMicrofluidic Analytical TechniquesAnimalsCytokinesImmunoassayMiceMicrofluidicsTumor Necrosis Factor-alphaCytokinesTumor Necrosis Factor-alphaAlzheimer's diseaseamyloid-beta phagocytosiscytokine secretiondigital immunoassaymicroglia

Identifiers

PMID34170616
PMCPMC8349899
OpenAlexW3173751787

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.