ArticleDevelopment (Cambridge, England)2021
Microfluidic-based imaging of complete Caenorhabditis elegans larval development.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- IndividualLife science alliance · 2026Article
- Tagging of C. elegans apoptosis activator EGL-1 BH3-only reveals CED-9 BCL-2-dependent mitochondrial localization and dynamic control of EGL-1 synthesis and degradation in vivo.Cell death and differentiation · 2026Article
- Transcriptional feedback targeting Wnt pathway components reveals hidden heterogeneity in Caenorhabditis elegans seam cell lineages.Genetics · 2026Article
- A conserved chronobiological complex times C. elegans development.The EMBO journal · 2025Article
- Automated Platforms inMicromachines · 2025Review
- Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo.Nature communications · 2025Article
- The C. elegans anchor cell: A model to elucidate mechanisms underlying invasion through basement membrane.Seminars in cell & developmental biology · 2024Review
- Prolonging somatic cell proliferation through constitutive hox gene expression in C. elegans.Nature communications · 2023Article
- Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis.Quantitative plant biology · 2023Article
- Surface acoustic wave microfluidics for repetitive and reversible temporary immobilization ofLab on a chip · 2022Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Tissue-specific inhibition of protein sumoylation uncovers diverse SUMO functions during C. elegans vulval development.PLoS genetics · 2022Article
- SALSA, a genetically encoded biosensor for spatiotemporal quantification of Notch signal transduction in vivo.Developmental cell · 2022Article
- Live-cell Imaging and Analysis of Germline Stem Cell Mitosis inBio-protocol · 2022Article
- Reciprocal EGFR signaling in the anchor cell ensures precise inter-organ connection during Caenorhabditis elegans vulval morphogenesis.Development (Cambridge, England) · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Several microfluidic-based methods for Caenorhabditis elegans imaging have recently been introduced. Existing methods either permit imaging across multiple larval stages without maintaining a stable worm orientation, or allow for very good immobilization but are only suitable for shorter experiments. Here, we present a novel microfluidic imaging method that allows parallel live-imaging across multiple larval stages, while maintaining worm orientation and identity over time. This is achieved through an array of microfluidic trap channels carefully tuned to maintain worms in a stable orientation, while allowing growth and molting to occur. Immobilization is supported by an active hydraulic valve, which presses worms onto the cover glass during image acquisition only. In this way, excellent quality images can be acquired with minimal impact on worm viability or developmental timing. The capabilities of the devices are demonstrated by observing the hypodermal seam and P-cell divisions and, for the first time, the entire process of vulval development from induction to the end of morphogenesis. Moreover, we demonstrate feasibility of on-chip RNAi by perturbing basement membrane breaching during anchor cell invasion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.