ArticleJournal of veterinary science2021
Molecular detection and characterization of ovine herpesvirus-2 using heminested PCR in Pakistan.
Article in Journal of veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Molecular prevalence and risk factors of ovine herpesvirus-2-associated malignant catarrhal fever in cattle from Riau Province, Indonesia: A cross-sectional nested polymerase chain reaction study.Veterinary world · 2026Article
- Ovine gammaherpesvirus 2 in asymptomatic water buffaloes (Bubalus bubalis) from Central-western Brazil and implications for infections in buffaloes worldwide.Tropical animal health and production · 2026Article
- Ovine gammaherpesvirus-2 associated manifestations with concomitant polioencephalomalacia, bovine alphaherpesvirus 1-related pneumonia, and caseous lymphadenitis in a sheep.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Serological Detection of Ovine Gammaherpesvirus 2 Antibodies in Dairy Farms from Southern Brazil.Microorganisms · 2024Article
- Molecular detection of ovine gammaherpesvirus 2 in free ranging wild boars (Sus scrofa) from Southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Viral Diseases in Water Buffalo (Animals : an open access journal from MDPI · 2024Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundMalignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2).
objectivesThis study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods.
methodsBlood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. The samples were subjected to heminested polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the OvHV-2 POL gene and the OvHV-2 ORF75 tegument protein gene.
resultsThe highest percentage of MCF positive samples was in sheep (13%), whereas goat, cattle, and buffalo had lower positive percentages, 11%, 9%, and 6.5%, respectively. Four OvHV-2-positive PCR products obtained from sheep samples were sequenced. The sequences obtained were submitted to the NCBI GenBank database (MK852173 for the POL gene; MK840962, MK852171, and MK852172 for the ORF75 tegument protein gene). Phylogenetic analysis revealed a close similarity of study sequences with those of worldwide samples.
conclusionsThis study is the first cross-sectional study on the prevalence and molecular detection of OvHV-2 in apparently healthy cattle and buffalo that could be carrying OvHV-2 acquired from OvHV-2-positive sheep and goats. The results indicate that OvHV-2 is circulating in Pakistan. Further studies are needed to characterize OvHV-2 and elucidate further its prevalence.
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