ArticleThe EMBO journal2021
Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- The C-terminal ZZ domain of the Drosophila ORB2 RNA-binding protein is required for spermatid individualization.G3 (Bethesda, Md.) · 2026Article
- Small HSPs at the crossroad between protein aggregation, autophagy and unconventional secretion: clinical implications and potential therapeutic opportunities in the context of neurodegenerative diseases.Frontiers in cell and developmental biology · 2025Review
- Dual roles of AtNBR1 in regulating selective autophagyAutophagy · 2024Article
- Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine.Oncology letters · 2024Article
- Two aminopeptidase I homologs convergently contribute to pathobiology of fungal entomopathogen Beauveria bassiana via divergent physiology-dependent autophagy pathways for vacuolar targeting.Journal of advanced research · 2024Article
- Pseudomonas aeruginosa biofilm exopolysaccharides: assembly, function, and degradation.FEMS microbiology reviews · 2023Review
- Autophagy genes in biology and disease.Nature reviews. Genetics · 2023Review
- Article
- Antibody screening reveals antigenic proteins involved inFrontiers in cellular and infection microbiology · 2023Article
- NBR1: The archetypal selective autophagy receptor.The Journal of cell biology · 2022Review
- Structural mechanism of protein recognition by the FW domain of autophagy receptor Nbr1.Nature communications · 2022Article
- The ZZ domain of HERC2 is a receptor of arginylated substrates.Scientific reports · 2022Article
- Fission Yeast Autophagy Machinery.Cells · 2022Review
- ZZ domains keep cytosol to vacuole delivery whiZZing along.The EMBO journal · 2021Article
- Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1.The EMBO journal · 2021Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In selective autophagy, cargo selectivity is determined by autophagy receptors. However, it remains scarcely understood how autophagy receptors recognize specific protein cargos. In the fission yeast Schizosaccharomyces pombe, a selective autophagy pathway termed Nbr1-mediated vacuolar targeting (NVT) employs Nbr1, an autophagy receptor conserved across eukaryotes including humans, to target cytosolic hydrolases into the vacuole. Here, we identify two new NVT cargos, the mannosidase Ams1 and the aminopeptidase Ape4, that bind competitively to the first ZZ domain of Nbr1 (Nbr1-ZZ1). High-resolution cryo-EM analyses reveal how a single ZZ domain recognizes two distinct protein cargos. Nbr1-ZZ1 not only recognizes the N-termini of cargos via a conserved acidic pocket, similar to other characterized ZZ domains, but also engages additional parts of cargos in a cargo-specific manner. Our findings unveil a single-domain bispecific mechanism of autophagy cargo recognition, elucidate its underlying structural basis, and expand the understanding of ZZ domain-mediated protein-protein interactions.
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