ArticleChemical science2021
Direct observation of ion emission from charged aqueous nanodrops: effects on gaseous macromolecular charging.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Asymmetry in Droplet Charge and HJACS Au · 2026Article
- Atmospheric Sampling Mass Spectrometers Activate and Ionize Neutral Water Microdroplets to MeV Energies and up to 200,000+ Charges: Implications for Water Stability and Unusual Chemistry in Microdroplets.ACS central science · 2025Article
- Adeno-associated virus serotype 9 structural heterogeneity and stability characterized by charge detection mass spectrometry.Molecular therapy. Methods & clinical development · 2025Article
- Coupling Drift Tube Ion Mobility Measurements with Charge Detection Mass Spectrometry for the Analysis of Virus-like Particles and Other Megadalton-Sized Ions.Analytical chemistry · 2025Article
- An Alternative Explanation for Ions Put Forth as Evidence for Abundant Hydroxyl Radicals Formed Due to the Intrinsic Electric Field at the Surface of Water Droplets.Analytical chemistry · 2025Article
- Single particle charge detection mass spectrometry enables molecular characterization of lipid nanoparticles and mRNA packaging.Journal of controlled release : official journal of the Controlled Release Society · 2025Article
- Spontaneous Fission of Charged Water Nanodrops: Unveiling the Stochastic Nature of Fission Pathways and Dynamics.Journal of the American Chemical Society · 2025Article
- Characterizing Monoclonal Antibody Aggregation Using Charge Detection Mass Spectrometry and Industry Standard Methods.Journal of the American Society for Mass Spectrometry · 2025Article
- A Source of the MysteriousACS central science · 2025Article
- Article
- Prolonged Trapping of Adeno-Associated Virus Capsids Reveals that Genome Packaging Affects Single-Ion Mass Spectrometry Measurements.Journal of the American Chemical Society · 2025Article
- Understanding the Formation Dynamics and Physical Properties of Nanocapsules Using Charge Detection Mass Spectrometry.ACS nano · 2025Article
- High-Throughput Single-Particle Characterization of Aggregation Pathways and the Effects of Inhibitors for Large (Megadalton) Protein Oligomers.Analytical chemistry · 2024Article
- Nanopore ion sources deliver individual ions of amino acids and peptides directly into high vacuum.Nature communications · 2024Article
- Charge Detection Mass Spectrometry Reveals Conformational Heterogeneity in Megadalton-Sized Monoclonal Antibody Aggregates.Journal of the American Chemical Society · 2024Article
- Article
- Single-Ion Mass Spectrometry for Heterogeneous and High Molecular Weight Samples.Journal of the American Chemical Society · 2024Review
- Ion emission from 1-10 MDa salt clusters: individual charge state resolution with charge detection mass spectrometry.The Analyst · 2024Article
- Combined Multiharmonic Frequency Analysis for Improved Dynamic Energy Measurements and Accuracy in Charge Detection Mass Spectrometry.Analytical chemistry · 2023Article
- Dynamics of Rayleigh Fission Processes in ∼100 nm Charged Aqueous Nanodrops.ACS central science · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mechanistic information about how gaseous ions are formed from charged droplets has been difficult to establish because direct observation of nanodrops in a size range relevant to gaseous macromolecular ion formation by optical or traditional mass spectrometry methods is challenging owing to their small size and heterogeneity. Here, the mass and charge of individual aqueous nanodrops between 1-10 MDa (15-32 nm diameter) with ∼50-300 charges are dynamically monitored for 1 s using charge detection mass spectrometry. Discrete losses of minimally solvated singly charged ions occur, marking the first direct observation of ion emission from aqueous nanodrops in late stages of droplet evaporation relevant to macromolecular ion formation in native mass spectrometry. Nanodrop charge depends on the identity of constituent ions, with pure water nanodrops charged slightly above the Rayleigh limit and aqueous solutions containing alkali metal ions charged progressively below the Rayleigh limit with increasing cation size. MS2 capsid ions (∼3.5 MDa; ∼27 nm diameter) are more highly charged from aqueous ammonium acetate than from its biochemically preferred, 100 mM NaCl/10 mM Na phosphate solution, consistent with ion emission reducing the nanodrop and resulting capsid charge. The extent of charging indicates that the capsid partially collapses inside the nanodrops prior to the charging and formation of the dehydrated gaseous ions. These results demonstrate that ion emission can affect macromolecular charging and that conformational changes to macromolecular structure can occur in nanodrops prior to the formation of naked gaseous ions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.