Evidence map›Paper›PMID 34168121›Full record

ArticleNature communications2021

Accurate localization microscopy by intrinsic aberration calibration.

Craig R Copeland, Craig D McGray, B Robert Ilic, Jon Geist, Samuel M Stavis

Abstract read
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
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  4. 3D Biological/Biomedical Image Registration with enhanced Feature Extraction and Outlier Detection.ACM-BCB ... ... : the ... ACM Conference on Bioinformatics, Computational Biology and Biomedicine. ACM Conference on Bioinformatics, Computational Biology and Biomedicine · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Craig R CopelandMicrosystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.ORCID http://orcid.org/0000-0002-3340-0006
Craig D McGrayQuantum Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.ORCID http://orcid.org/0000-0002-4613-1925
B Robert IlicMicrosystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.ORCID http://orcid.org/0000-0003-2504-4045
Jon GeistQuantum Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.
Samuel M StavisMicrosystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA. samuel.stavis@nist.gov.ORCID http://orcid.org/0000-0002-7108-1405

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A standard paradigm of localization microscopy involves extension from two to three dimensions by engineering information into emitter images, and approximation of errors resulting from the field dependence of optical aberrations. We invert this standard paradigm, introducing the concept of fully exploiting the latent information of intrinsic aberrations by comprehensive calibration of an ordinary microscope, enabling accurate localization of single emitters in three dimensions throughout an ultrawide and deep field. To complete the extraction of spatial information from microscale bodies ranging from imaging substrates to microsystem technologies, we introduce a synergistic concept of the rigid transformation of the positions of multiple emitters in three dimensions, improving precision, testing accuracy, and yielding measurements in six degrees of freedom. Our study illuminates the challenge of aberration effects in localization microscopy, redefines the challenge as an opportunity for accurate, precise, and complete localization, and elucidates the performance and reliability of a complex microelectromechanical system.

Identifiers

PMID34168121
PMCPMC8225824

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.