Evidence map›Paper›PMID 34167469›Full record

ArticleBMC genomics2021

Morphological changes and functional circRNAs screening of rabbit skeletal muscle development.

Qi Zheng, Cuiyun Zhu, Jing Jing, Yinghui Ling, Shuaiqi Qin, Jiao Wang, Lisha Zha, Ya Liu, Fugui Fang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Qi Zheng *College of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Cuiyun Zhu *College of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Jing JingCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Yinghui LingCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China. lingyinghui@ahau.edu.cn.
Shuaiqi QinCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Jiao WangCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Lisha ZhaCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China. zhalisha@ahau.edu.cn.
Ya LiuCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Fugui FangCollege of Animal Science and Technology, Anhui Agricultural University, Anhui, Hefei, P.R. China.
Anhui Agricultural University · CN

Funding

National Natural Science Foundation of China 31772566the Anhui Province Academic and Technology Leader Reserve Talent Project 2019H206the Special fund for Anhui provincial central government to guide local science and technology development 202007d06020005
6 · The paper itself

Abstract

backgroundThe temporal expression pattern of circular RNAs (circRNAs) across developmental stages is essential for skeletal muscle growth and functional analysis. However, there are few analyses on the potential functions of circRNAs in rabbit skeletal muscle development.

resultsInitially, the paraffin sections showed extremely significant differences in the diameter, number, area and density of skeletal muscle fibers of the fetus, child, adult rabbit hind legs (P < 0.01). Then, RNA-seq libraries of these three stages were constructed. A total of 481 differentially expressed circRNAs (DE-circRNAs) and 5,658 differentially expressed genes (DEGs) were identified. Subsequently, DE-circRNAs, whose host genes were DEGs or non-DEGs, were analyzed by GO respectively. In the fetus vs. child group, up-regulated DE-circRNAs (whose host genes were DEGs) were related to muscle fiber structure, and down-regulated ones were related to mitosis. The up-regulated DE-circRNAs (whose host genes were non-DEGs) were involved in enzyme activity, methylation and glycosylation, and the down-regulated ones were involved in mitosis and catabolism. In the fetus vs. adult group, the up-regulated DE-circRNAs (whose host genes were DEGs) were related to skeletal muscle basic structure, and the down-regulated ones were also associated with cell proliferation. But the up-regulated DE-circRNAs (whose host genes were non-DEGs) were connected with regulation of histone ubiquitination, chromatin and organelles. The down-regulated DE-circRNAs were connected with the catabolism processes. In addition, novel_circ_0022663 and novel_circ_0005489, which might have coding potential, and novel_circ_0004210 and novel_circ_0001669, which might have miRNA sponge capability, were screened out.

conclusionsIn this study, hind leg muscles of fetus, child and adult rabbits were collected for paraffin section and RNA-seq to observe the structural changes of skeletal muscle and obtain circRNA expression profiles at different stages. These data provided a catalog of circRNAs related to muscle development in New Zealand rabbits, allowing us to better understand the functional transitions in mammalian muscle development.

Indexed as

MicroRNAsRNA, CircularAnimalsMuscle DevelopmentRabbitsRNA-SeqMicroRNAsRNA, CircularCircRNAMorphologyRabbitRNA-seqSkeletal muscle

Identifiers

PMID34167469
PMCPMC8223307
OpenAlexW3175532813

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.