ArticleeLife2021
Different translation dynamics of β- and γ-actin regulates cell migration.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- ACTG1 facilitates proliferation and epithelial-mesenchymal transition-mediated metastasis via COL21A1 in gastric cancer.iScience · 2026Article
- A microexon in Arp2 alters tissue-specific Arp2/3-generated actin structures.bioRxiv : the preprint server for biology · 2025Article
- Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis.NPJ Parkinson's disease · 2025Article
- Amino acid-level differences in alpha-tubulin sequences are uniquely required for meiosis.Molecular biology of the cell · 2025Article
- All Probes Plasmids (APPs) for multicolor and long-term tracking of single-mRNA translation dynamics.Molecular biology of the cell · 2025Article
- The divergent intron-containing actin in sponge morphogenetic processes.NAR genomics and bioinformatics · 2025Article
- G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.Cell reports · 2025Article
- Structural and functional mechanisms of actin isoforms.The FEBS journal · 2025Review
- Translation Dynamics of Single mRNAs in Live Cells.Annual review of biophysics · 2024Review
- IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms.PLoS biology · 2024Article
- Incorporating mutational heterogeneity to identify genes that are enriched for synonymous mutations in cancer.BMC bioinformatics · 2023Article
- Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function.Nature communications · 2023Article
- Identification and characterization of ARID1A-interacting proteins in renal tubular cells and their molecular regulation of angiogenesis.Journal of translational medicine · 2023Article
- mRNA location and translation rate determine protein targeting to dual destinations.Molecular cell · 2023Article
- Impact of Protein NLife (Basel, Switzerland) · 2023Review
- Myosin and [Formula: see text]-actinin regulation of stress fiber contractility under tensile stress.Scientific reports · 2023Article
- Recycling of the actin monomer pool limits the lifetime of network turnover.The EMBO journal · 2023Article
- Article
- Structural insights into actin isoforms.eLife · 2023Article
- The RNA-Binding Protein SMN as a Novel Player in Laryngeal Squamous Cell Carcinoma.International journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
β- and γ-cytoplasmic actins are ubiquitously expressed in every cell type and are nearly identical at the amino acid level but play vastly different roles in vivo. Their essential roles in embryogenesis and mesenchymal cell migration critically depend on the nucleotide sequences of their genes, rather than their amino acid sequences; however, it is unclear which gene elements underlie this effect. Here we address the specific role of the coding sequence in β- and γ-cytoplasmic actins' intracellular functions, using stable polyclonal populations of immortalized mouse embryonic fibroblasts with exogenously expressed actin isoforms and their 'codon-switched' variants. When targeted to the cell periphery using β-actin 3'UTR; β-actin and γ-actin have differential effects on cell migration. These effects directly depend on the coding sequence. Single-molecule measurements of actin isoform translation, combined with fluorescence recovery after photobleaching, demonstrate a pronounced difference in β- and γ-actins' translation elongation rates in cells, leading to changes in their dynamics at focal adhesions, impairments in actin bundle formation, and reduced cell anchoring to the substrate during migration. Our results demonstrate that coding sequence-mediated differences in actin translation play a key role in cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.