ArticleChemical science2021
A straightforward methodology to overcome solubility challenges for N-terminal cysteinyl peptide segments used in native chemical ligation.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Total Chemical Synthesis of Interleukin-15 and Interleukin-2: Taming Protein Hydrophobicity and Aggregation by a Versatile Solubilizing Strategy.Angewandte Chemie (International ed. in English) · 2026Article
- Proximity-driven acceleration of challenging solid-phase peptide couplings.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Advances in the chemical synthesis of human proteoforms.Science China. Life sciences · 2025Review
- Selective Activation of Peptide-Thioester Precursors for Templated Native Chemical Ligations.Angewandte Chemie (International ed. in English) · 2025Article
- A Versatile "Synthesis Tag" (SynTag) for the Chemical Synthesis of Aggregating Peptides and Proteins.Journal of the American Chemical Society · 2024Article
- Uncovering a Latent Bioactive Interleukin-6 Glycoform.Angewandte Chemie (International ed. in English) · 2024Article
- A cysteine-specific solubilizing tag strategy enables efficient chemical protein synthesis of difficult targets.Chemical science · 2024Article
- Synthesis and applications of mirror-image proteins.Nature reviews. Chemistry · 2023Review
- Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily.International journal of molecular sciences · 2022Article
- Enabling chemical protein (semi)synthesisChemical science · 2022Article
- A glutamic acid-based traceless linker to address challenging chemical protein syntheses.Organic & biomolecular chemistry · 2021Article
- Insights into the Mechanism and Catalysis of Peptide Thioester Synthesis by Alkylselenols Provide a New Tool for Chemical Protein Synthesis.Molecules (Basel, Switzerland) · 2021Article
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Authors and funding
5 authors.
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Abstract
One of the main limitations encountered during the chemical synthesis of proteins through native chemical ligation (NCL) is the limited solubility of some of the peptide segments. The most commonly used solution to overcome this problem is to derivatize the segment with a temporary solubilizing tag. Conveniently, the tag can be introduced on the thioester segment in such a way that it is removed concomitantly with the NCL reaction. We herein describe a generalization of this approach to N-terminal cysteinyl segment counterparts, using a straightforward synthetic approach that can be easily automated from commercially available building blocks, and applied it to a well-known problematic target, SUMO-2.
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