ArticleChemical science2021
Self-programmed enzyme phase separation and multiphase coacervate droplet organization.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Liquid-Liquid Phase Separation-Enhanced Multienzyme Catalysis: Mechanisms and Applications.ChemSusChem · 2026Review
- Dynamic and Catalytic Multiphase Coacervates.Biomacromolecules · 2026Article
- Adaptive Coacervate Microreactors with Broad pH/Thermal Tolerance for Reaction Acceleration.JACS Au · 2026Article
- Article
- Preservation of Complex Multiphase Architectures in Polymer-Based Artificial Cells by Photo-Crosslinking.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Complex Coacervate Emulsions as a Strategy to Stabilize Enzymes for Catalysis in Organic Solvents.ACS macro letters · 2026Article
- Transient pH changes drive vacuole formation in enzyme-polymer condensates.Nature chemical engineering · 2026Article
- Dual-Stimulus Programmed Multiphase Separation and Organization in Coacervate Droplets.Angewandte Chemie (International ed. in English) · 2025Article
- Adaptive ATP-induced molecular condensation in membranized protocells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Phase-Separated Spiropyran Coacervates as Dual-Wavelength-Switchable Reactive Oxygen Generators.Angewandte Chemie (International ed. in English) · 2025Article
- Polyelectrolyte-Carbon Dot Complex Coacervation.ACS macro letters · 2025Article
- Pyrite as a catalyst for the emergence of multiphase primitive cells.Frontiers in microbiology · 2025Article
- Biomimetic Materials to Fabricate Artificial Cells.Chemical reviews · 2024Review
- Enzymatic Reaction Network-Driven Polymerization-Induced Transient Coacervation.Angewandte Chemie (International ed. in English) · 2024Article
- Coacervate Droplets as Biomimetic Models for Designing Cell-Like Microreactors.Macromolecular rapid communications · 2024Review
- Periodic Alignment of Binary Droplets via a Microphase Separation of a Tripolymer Solution under Tubular Confinement.ACS macro letters · 2024Article
- Understanding the Impacts of Molecular and Macromolecular Crowding Agents on Protein-Polymer Complex Coacervates.Biomacromolecules · 2023Article
- Article
- Designing negative feedback loops in enzymatic coacervate droplets.Chemical science · 2023Article
- Non-enzymatic oligonucleotide ligation in coacervate protocells sustains compartment-content coupling.Nature communications · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membraneless organelles are phase-separated droplets that are dynamically assembled and dissolved in response to biochemical reactions in cells. Complex coacervate droplets produced by associative liquid-liquid phase separation offer a promising approach to mimic such dynamic compartmentalization. Here, we present a model for membraneless organelles based on enzyme/polyelectrolyte complex coacervates able to induce their own condensation and dissolution. We show that glucose oxidase forms coacervate droplets with a cationic polysaccharide on a narrow pH range, so that enzyme-driven monotonic pH changes regulate the emergence, growth, decay and dissolution of the droplets depending on the substrate concentration. Significantly, we demonstrate that time-programmed coacervate assembly and dissolution can be achieved in a single-enzyme system. We further exploit this self-driven enzyme phase separation to produce multiphase droplets
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Registered trials
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