ArticleChemical science2021
Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Riboswitch-controlled lipid conversion enables functional membrane asymmetry in artificial cells.Communications biology · 2026Article
- Small amphiphilic DNA for programmable transmembrane signaling and amplification.bioRxiv : the preprint server for biology · 2025Article
- Passive Membrane Transport Analysis of Drug Mixtures.Analytical chemistry · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- High-throughput single biomarker identification using droplet nanopore.Chemical science · 2024Article
- Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations.Communications chemistry · 2024Article
- Cell-Free Synthesis Goes Electric: Dual Optical and Electronic Biosensor via Direct Channel Integration into a Supported Membrane Electrode.ACS synthetic biology · 2023Article
- How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels.International journal of molecular sciences · 2022Review
- Challenges and opportunities in achieving the full potential of droplet interface bilayers.Nature chemistry · 2022Review
- Hydrogels as functional components in artificial cell systems.Nature reviews. Chemistry · 2022Review
- Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.Nature communications · 2022Article
- Enhancing membrane-based soft materials with magnetic reconfiguration events.Scientific reports · 2022Article
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Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between membrane separated compartments. This has been demonstrated with droplet interface bilayers (DIBs) using several different membrane proteins combined with electrical, mechanical, and/or chemical activators. Here we report the activation of the bacterial mechanosensitive channel of large conductance (MscL) in a dioleoylphosphatidylcholine:dioleoylphosphatidylglycerol DIB by controlling membrane asymmetry. We show using electrical measurements that the incorporation of lysophosphatidylcholine (LPC) into one of the bilayer leaflets triggers MscL gating in a concentration-dependent manner, with partial and full activation observed at 10 and 15 mol% LPC respectively. Our findings could inspire the design of new minimal tissues where flux pathways are dynamically defined by lipid composition.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.