Evidence map›Paper›PMID 34163682›Full record

ArticleChemical science2021

Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution.

Qi Zhang, Yunlong Shao, Boye Li, Yuanyuan Wu, Jingying Dong, Dongtang Zhang, Yanan Wang, Yong Yan, Xiayan Wang, Qiaosheng Pu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Qi ZhangCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Yunlong ShaoCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Boye LiCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Yuanyuan WuCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Jingying DongCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Dongtang ZhangCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.ORCID https://orcid.org/0000-0002-2698-6694
Yanan WangCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Yong YanCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Xiayan WangCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.ORCID https://orcid.org/0000-0003-1735-0077
Qiaosheng PuDepartment of Chemistry, Lanzhou University Lanzhou Gansu 730000 China puqs@lzu.edu.cn.ORCID https://orcid.org/0000-0002-5241-7196
Guangsheng GuoCenter of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology Beijing 100124 China xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The analysis of single living cells, including intracellular delivery and extraction, is essential for monitoring their dynamic biochemical processes and exploring intracellular heterogeneity. However, owing to the 2D view in bright-field microscopy and optical distortions caused by the cell shape and the variation in the refractive index both inside and around the cells, achieving spatially undistorted imaging for high-precision manipulation within a cell is challenging. Here, an accurate and visual system is developed for single-cell spatial manipulation by correcting the aberration for simultaneous bright-field triple-view imaging. Stereo information from the triple view enables higher spatial resolution that facilitates the precise manipulation of single cells. In the bright field, we resolved the spatial locations of subcellular structures of a single cell suspended in a medium and measured the random spatial rotation angle of the cell with a precision of ±5°. Furthermore, we demonstrated the visual manipulation of a probe to an arbitrary spatial point of a cell with an accuracy of <1 pixel. This novel system is more accurate and less destructive for subcellular content extraction and drug delivery.

Identifiers

PMID34163682
PMCPMC8179525

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.