Evidence map›Paper›PMID 34162966›Full record

ArticleScientific reports2021

A method to analyze time expression profiles demonstrated in a database of chili pepper fruit development.

Christian Escoto-Sandoval, Alan Flores-Díaz, M Humberto Reyes-Valdés, Neftalí Ochoa-Alejo, Octavio Martínez

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.5field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Christian Escoto-SandovalCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Unidad de Genómica Avanzada (Langebio), Irapuato, Guanajuato, 36824, Mexico.
Alan Flores-DíazCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Unidad de Genómica Avanzada (Langebio), Irapuato, Guanajuato, 36824, Mexico.
M Humberto Reyes-ValdésDepartment of Plant Breeding, Universidad Autónoma Agraria Antonio Narro, Saltillo, Coahuila, 25315, Mexico.
Neftalí Ochoa-AlejoCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Departamento de Ingeniería Genética, Unidad Irapuato, Irapuato, Guanajuato, 36824, Mexico.
Octavio MartínezCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Unidad de Genómica Avanzada (Langebio), Irapuato, Guanajuato, 36824, Mexico. octavio.martinez@cinvestav.mx.
Center for Research and Advanced Studies of the National Polytechnic Institute · MXUniversidad Autónoma Agraria Antonio Narro · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA-Seq experiments allow genome-wide estimation of relative gene expression. Estimation of gene expression at different time points generates time expression profiles of phenomena of interest, as for example fruit development. However, such profiles can be complex to analyze and interpret. We developed a methodology that transforms original RNA-Seq data from time course experiments into standardized expression profiles, which can be easily interpreted and analyzed. To exemplify this methodology we used RNA-Seq data obtained from 12 accessions of chili pepper (Capsicum annuum L.) during fruit development. All relevant data, as well as functions to perform analyses and interpretations from this experiment, were gathered into a publicly available R package: "Salsa". Here we explain the rational of the methodology and exemplify the use of the package to obtain valuable insights into the multidimensional time expression changes that occur during chili pepper fruit development. We hope that this tool will be of interest for researchers studying fruit development in chili pepper as well as in other angiosperms.

Indexed as

Gene Expression Regulation, PlantAlgorithmsCapsicumDatabases, GeneticData MiningDatasets as TopicFruitRNA, PlantRNA-SeqTime FactorsRNA, Plant

Identifiers

PMID34162966
PMCPMC8222228
OpenAlexW3174076796

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.