ArticleScientific reports2021
Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Two-step workflow integrating automatic registration and manual refinement for the accurate alignment of serial histological sections in 3D reconstruction.Journal of anatomy · 2026Article
- Thin-Adipose Compartment at the Colonic Mesentery-Perirenal Fat Interface: Histological and Three-Dimensional Morphological Studies.International journal of urology : official journal of the Japanese Urological Association · 2026Article
- 3D Imaging of Gene Expression Domains in the Mouse Heart.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Precise MRI-Histology Coregistration of Paraffin-Embedded Tissue with Blockface Imaging.bioRxiv : the preprint server for biology · 2025Article
- Precise MRI-histology coregistration of paraffin-embedded tissue with blockface imaging.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- Multiscale Three-Dimensional Evaluation and Analysis of Murine Lung Vasculature From Macro- to Micro-Structural Level.Pulmonary circulation · 2025Article
- Development of a heat-stable alkaline phosphatase reporter system for cis-regulatory analysis and its application to 3D digital imaging of Xenopus embryonic tissues.Development, growth & differentiation · 2024Article
- Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science.Anatomical science international · 2023Review
- Novel combination method of wide-range serial sectioning and 3D reconstruction visualizing both macro-level dynamics and micro-level interactions in an attempt to analyze the female pelvic floor.Anatomical science international · 2023Review
- Visualising the effect of freezing on the vascular system of wheat in three dimensions by in-block imaging of dye-infiltrated plants.Journal of microscopy · 2022Article
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Authors and funding
11 authors.
Funding
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Abstract
Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the morphological analyses of various biological specimens, and its use is expanding. However, the conventional CoMBI system utilizes a cryostat, which limits its compatibility to only frozen blocks and the resolution of the block-face image. We developed a new CoMBI system that can be applied to not only frozen blocks but also paraffin blocks, and it has an improved magnification for block-face imaging. The new system, called CoMBI-S, comprises sliding-type sectioning devices and imaging devices, and it conducts block slicing and block-face imaging automatically. Sections can also be collected and processed for microscopy as required. We also developed sample preparation methods for improving the qualities of the block-face images and 3D rendered volumes. We successfully obtained correlative 3D datasets and 2D microscopic images of zebrafish, mice, and fruit flies, which were paraffin-embedded or frozen. In addition, the 3D datasets at the highest magnification could depict a single neuron and bile canaliculus.
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