ArticleScientific reports2021
Hypoxia and heat stress affect epithelial integrity in a Caco-2/HT-29 co-culture.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 70 citations in OpenAlex.
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- Chronic heat stress compromises egg production and quality parameters through changes in blood biochemistry and uterine gene expression in laying hens raised under cage-free environment.Frontiers in physiology · 2026Article
- Research progress on the mechanisms and intervention strategies of microbiota-gut-brain axis dysfunction under heat stress.Frontiers in physiology · 2026Review
- From mechanisms to therapeutics: molecular insights into gastrointestinal injury under high-altitude hypoxia.Frontiers in microbiology · 2026Review
- Gut microbiota modulation in the prevention and treatment of heat stroke.Frontiers in immunology · 2026Review
- A comparative study of organoid-derived and cell line-derived intestinal epithelial models.PloS one · 2026Article
- Molecular signatures and machine learning driven stress biomarkers for rainbow trout aquaculture and climate adaptation.Scientific reports · 2025Article
- Comprehensive Evaluation of the Genotoxic Potential of Food Additive Titanium Dioxide in Human Intestinal Cell Systems.International journal of molecular sciences · 2025Article
- A simple, efficient technique to make colon crypt gels for 3D models.3D printing and additive manufacturing · 2025Article
- Fecal microbiota transplantation alleviated heat-induced colonic tissue damage, epithelial apoptosis, and oxidative stress.Applied and environmental microbiology · 2025Article
- Gamma-aminobutyric acid attenuates cortisol-induced damage in human colorectal adenocarcinoma cells via Nrf2 signaling.Die Naturwissenschaften · 2025Article
- Unique Athletic Gut Microbiomes and Their Role in Sports Performance: A Narrative Review.Journal of human kinetics · 2025Review
- Geometrically Controlled WNT Activation Drives Intestinal Morphogenesis.Advanced healthcare materials · 2025Article
- Bioactive Properties and Phenolic Profile of Bioaccessible and Bioavailable Fractions of Red Radish Microgreens After In Vitro Digestion.Molecules (Basel, Switzerland) · 2025Article
- Molecular and phenotypic characterization of 5-FU resistant colorectal cancer cells: toward enrichment of cancer stem cells.Cancer cell international · 2025Article
- Heat Stress Influences Immunity ThroughAnimals : an open access journal from MDPI · 2025Article
- Major Oxidative and Antioxidant Mechanisms During Heat Stress-Induced Oxidative Stress in Chickens.Antioxidants (Basel, Switzerland) · 2025Review
- Oxidative Stress by HAntioxidants (Basel, Switzerland) · 2025Article
- Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets.International journal of molecular sciences · 2025Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypoxia and hyperthermia, which can be induced by high environmental temperature or strenuous exercise, are two common stressors that affect intestinal epithelial integrity and lead to multiple clinical symptoms. In this study, we developed an in-vitro intestinal monolayer model using two human colonic epithelial cell lines, Caco-2 and HT-29, co-cultured in Transwell inserts, and investigated the effects of heat treatment and/or hypoxia on the epithelial barrier function. The monolayer with a ratio of 9:1 (Caco-2:HT-29) showed high trans-epithelial electrical resistance (TEER), low Lucifer Yellow permeability and high mucin production. Hyperthermia and/or hypoxia exposure (2 h) triggered heat shock and oxidative stress responses. HSP-70 and HSF-1 protein levels were up-regulated by hyperthermia, which were further enhanced when hyperthermia was combined with hypoxia. Increased HIF-1α protein expression and Nrf2 nuclear translocation was only caused by hypoxia. Hyperthermia and/or hypoxia exposure disrupted the established monolayer by increasing paracellular permeability, decreasing ZO-1, claudin-3 and occludin protein/mRNA expression, while enhancing E-cadherin protein expression. Tight junction protein distribution in the monolayer was also modulated by the hyperthermia and/or hypoxia exposure. In addition, transcription levels of mucin genes, MUC-2 and MUC-5AC, were increased after 2 h of hyperthermia and/or hypoxia exposure. In conclusion, this Caco-2/HT-29 cell model is valid and effective for studying detrimental effects of hyperthermia and/or hypoxia on intestinal barrier function and related heat shock and oxidative stress pathways and can be used to investigate possible interventions to reverse hyperthermia and/or hypoxia-induced intestinal epithelial injury.
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