ArticleGenome biology2021
Using high-throughput multiple optical phenotyping to decipher the genetic architecture of maize drought tolerance.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Advancing crop improvement through GWAS and beyond in mung bean.Frontiers in plant science · 2024Pooled it
- Temporal phenotypic responses of early vegetative maize to heat, drought, and combined stresses.The Plant journal : for cell and molecular biology · 2026Article
- Review
- Advances in root phenotyping: high-throughput imaging, computational tools, and integrative approaches for crop improvement.Journal of experimental botany · 2026Review
- Advances in genomics-driven genetic decoding and genomic design breeding in tomato.Horticulture research · 2026Article
- Integrating high-throughput phenomics and GWAS unravels the HaCBF4-Horticulture research · 2026Article
- Omics-driven plant breeding through phenomics-enviromics crosstalk.Nature communications · 2026Review
- Multi-Omics Dissection of Drought Stress Responses in Crops: From Molecular Regulatory Networks to Climate-Resilient Breeding Applications.International journal of molecular sciences · 2026Review
- Unraveling plant phenotype to genotype associations with daily hyperspectral traits inPlant phenomics (Washington, D.C.) · 2026Article
- Genome-wide association study reveals the genetic architecture of drought tolerance in maize using yield-based resistance indices and transcriptomic evidence.The plant genome · 2026Article
- Multi-locus GWAS uncovers favorable alleles and candidate genes underlying water stress response in chrysanthemum.BMC plant biology · 2026Article
- Genetic dynamics drive maize growth and breeding.Genome biology · 2026Article
- Genomic basis underlying alternative transcripts-mediated drought tolerance in maize.Genome biology · 2026Article
- Genome-wide association mapping and candidate genes analysis of high-throughput image descriptors for wheat frost tolerance.Stress biology · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- A pangenome of maize provides genetic insights into drought resistance.Nature genetics · 2025Article
- A high-throughput phenotyping dataset for GWAS analysis of maize under combined drought and heat stress.Data in brief · 2025Article
- Genetic variation at transcription factor binding sites largely explains phenotypic heritability in maize.Nature genetics · 2025Article
- PhenoRob-F: An autonomous ground-based robot for high-throughput phenotyping of field crops.Plant phenomics (Washington, D.C.) · 2025Article
- Review
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Authors and funding
24 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDrought threatens the food supply of the world population. Dissecting the dynamic responses of plants to drought will be beneficial for breeding drought-tolerant crops, as the genetic controls of these responses remain largely unknown.
resultsHere we develop a high-throughput multiple optical phenotyping system to noninvasively phenotype 368 maize genotypes with or without drought stress over a course of 98 days, and collected multiple optical images, including color camera scanning, hyperspectral imaging, and X-ray computed tomography images. We develop high-throughput analysis pipelines to extract image-based traits (i-traits). Of these i-traits, 10,080 were effective and heritable indicators of maize external and internal drought responses. An i-trait-based genome-wide association study reveals 4322 significant locus-trait associations, representing 1529 quantitative trait loci (QTLs) and 2318 candidate genes, many that co-localize with previously reported maize drought responsive QTLs. Expression QTL (eQTL) analysis uncovers many local and distant regulatory variants that control the expression of the candidate genes. We use genetic mutation analysis to validate two new genes, ZmcPGM2 and ZmFAB1A, which regulate i-traits and drought tolerance. Moreover, the value of the candidate genes as drought-tolerant genetic markers is revealed by genome selection analysis, and 15 i-traits are identified as potential markers for maize drought tolerance breeding.
conclusionOur study demonstrates that combining high-throughput multiple optical phenotyping and GWAS is a novel and effective approach to dissect the genetic architecture of complex traits and clone drought-tolerance associated genes.
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