ArticlePloS one2021
Resistance mechanisms and fitness of pyraclostrobin-resistant isolates of Lasiodiplodia theobromae from mango orchards.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 24 citations in OpenAlex.
- Etiology, Pathogenicity, and Fungicide Management of Pigeonpea Shoot Dieback Caused byJournal of fungi (Basel, Switzerland) · 2026Article
- Baseline Sensitivity of Botryosphaeria spp. Isolated from Apples to Pyraclostrobin in Korea.The plant pathology journal · 2025Article
- Article
- Identification and Characterization of Diplodia parva and Diplodia crataegicola Causing Black Rot of Chinese Quince.The plant pathology journal · 2023Article
- Ternary Mixture of Azoxystrobin, Boscalid and Pyraclostrobin Disrupts the Gut Microbiota and Metabolic Balance of Honeybees (International journal of molecular sciences · 2023Article
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5 authors at 1 institution in 1 country.
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Abstract
backgroundStem-end rot, caused by Lasiodiplodia theobromae (Pat.) Griffon & Maubl is a serious postharvest disease in mango. In China, a high prevalence of the QoI fungicides resistance has been reported in the last decade. The study aimed to discuss factors determining rapid development of pyraclostrobin-resistance and its resistance mechanisms.
methodsTo determine the resistance stability and fitness of pyraclostrobin resistance in L. theobromae, three phenotypes of pyraclostrobin resistance were compared and analyzed for the EC50 values, mycelial growth, virulence and temperature sensitivity and osmotic stress sensitivity. The relative conductivity and enzyme activities of different phenotypes were compared under fungicide stress to explore possible biochemical mechanisms of pyraclostrobin resistance in L. theobromae. The Cytb gene sequences of different phenotypes were analysed.
resultsAll isolates retained their original resistance phenotypes during the 10 subcultures on a fungicide-free PDA, factor of sensitivity change (FSC) was approximately equal to 1. The resistance-pyraclostrobin of the field isolates should be relatively stable. Two pyraclostrobin-resistant phenotypes shared similar mycelial growth, virulence and temperature sensitivity with pyraclostrobin-sensitive phenotype. After treated by pyraclostrobin, the relative conductivity of the sensitive phenotype was significantly increased. The time of Pyr-R and Pyr-HR reached the most conductivity was about 8-10 times than that of Pyr-S, the time for the maximum value appearance showed significant differences between sensitive and resistant phenotypes. The activities of Glutathione S-transferase (GST), catalase (CAT) and peroxidase (POD) of Pyr-HR were 1.78, 5.45 and 1.65 times respectively, significantly higher than that of Pyr-S after treated by 200 mg/l pyraclostrobin.
conclusionThe results showed that the pyraclostrobin-resistant phenotypes displayed high fitness and high-risk. The nucleotide sequences were identical among all pyraclostrobin-resistant and -sensitive isolates. The pyraclostrobin resistance was not attributable to Cytb gene alterations, there may be some of other resistance mechanisms. Differential response of enzyme activity and cell membrane permeability were observed in resistant- and sensitive-isolates suggesting a mechanism of metabolic resistance.
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