ArticleProceedings of the National Academy of Sciences of the United States of America2021
Long-read sequencing revealed cooccurrence, host range, and potential mobility of antibiotic resistome in cow feces.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- Exploring the Oral Resistome: From Metagenomics to Precision Oral Health.Microorganisms · 2026Review
- Evolution, Mechanisms, and Therapeutic Implications of Mobile Tetracycline Destructases.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Metagenomics for antimicrobial resistance: from resistome surveillance to mechanistic inference.Journal of bacteriology · 2026Review
- Gut Microbiomes of Rainbow Trout and Atlantic Salmon: Nutritional Modulation, Mucosal Immunity, and Resistome Risk.Biology · 2026Review
- A review of computational approaches for metagenomics by long-read sequencing.Science China. Life sciences · 2026Review
- Captive breeding of specialty animals represents an overlooked yet critical reservoir for spreading antibiotic resistance genes.The ISME journal · 2026Article
- Soil-borne legacy facilitates the dissemination of antibiotic resistance genes in soil-plant continua.iMeta · 2025Article
- Article
- Effect of different bulking agents on the quality, microbial community structure and metabolic functions during human feces composting in foam composting device.Frontiers in microbiology · 2025Article
- Effects of dietary zinc on the gut microbiome and resistome of the gestating cow and neonatal calf.Animal microbiome · 2024Article
- Roadmap to tackle antibiotic resistance in the environment under the One Health framework.mLife · 2023Article
- Polluted wetlands contain multidrug-resistance plasmids encoding CTX-M-type extended-spectrum β-lactamases.Plasmid · 2023Article
- Strategies and tools in illumina and nanopore-integrated metagenomic analysis of microbiome data.iMeta · 2023Review
- Characterization of the gut microbiome and resistome of Galapagos marine iguanas (Amblyrhynchus cristatus) from uninhabited islands.Animal microbiome · 2022Article
- Metagenomic assembly reveals hosts and mobility of common antibiotic resistome in animal manure and commercial compost.Environmental microbiome · 2022Article
- Metagenomic Characterization of Resistance Genes in Deception Island and Their Association with Mobile Genetic Elements.Microorganisms · 2022Article
- Calves as Main Reservoir of Antibiotic Resistance Genes in Dairy Farms.Frontiers in public health · 2022Article
- Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review.Frontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While it is well recognized that the environmental resistome is global, diverse, and augmented by human activities, it has been difficult to assess risk because of the inability to culture many environmental organisms, and it is difficult to evaluate risk from current sequence-based environmental methods. The four most important criteria to determine risk are whether the antibiotic-resistance genes (ARGs) are a complete, potentially functional complement; if they are linked with other resistances; whether they are mobile; and the identity of their host. Long-read sequencing fills this important gap between culture and short sequence-based methods. To address these criteria, we collected feces from a ceftiofur-treated cow, enriched the samples in the presence of antibiotics to favor ARG functionality, and sequenced long reads using Nanopore and PacBio technologies. Multidrug-resistance genes comprised 58% of resistome abundance, but only 0.8% of them were plasmid associated; fluroquinolone-, aminoglycoside-, macrolide-lincosamide-streptogramin (MLS)-, and β-lactam-resistance genes accounted for 2.7 to 12.3% of resistome abundance but with 19 to 78% located on plasmids. A variety of plasmid types were assembled, some of which share low similarity to plasmids in current databases. Enterobacteriaceae were dominant hosts of antibiotic-resistant plasmids; physical linkage of extended-spectrum β-lactamase genes (
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.